Human Epiregulin

• 产品编号：CST-5494SC      品牌：CST       原厂货号：5494SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > EGF 表皮生长因子
• 应用分类：

描述：

Cell Signaling Technology的人源重组蛋白Epiregulin(hEpiregulin) 63位缬氨酸-108位亮氨酸（编号#NP_001423）在E.coli中表达。基于氨基酸测序，大于50%的重组人源Epiregulin在N氨基末端63位缬氨酸(MVSIT)有一个甲基化，分子量为5406。剩余部分的重组人源Epiregulin起始于63位缬氨酸(VSITK)。DTT还原和非还原蛋白游离物为5kDa多肽。6 μg还原和未还原的人源重组Epiregulin由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%。重组人源Epiregulin的生物活性由MCF-10A细胞增殖实验测定，每批产品的ED50在0.10-0.85 ng/ml之间。每1 μg hEpiregulin溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的经0.22 μm滤器过滤的溶液后冻干带有载体。经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物无载体。Epiregulin是EGF家族成员，它高表达于各种特异肿瘤细胞、胎盘细胞和巨噬细胞中[1,2]。Epiregulin诱导角质化细胞和角膜上皮细胞的增殖，卵母细胞成熟，并在孕期中的胚胎植入等有功能[3-5]。活性的可溶性epiregulin蛋白由跨膜前体经蛋白酶解加工产生。epiregulin是EGF家族成员中唯一既能和ErbB1/HER1结合又能和ErbB4/HER4 受体结合的蛋白。Epiregulin的结合诱导受体同源二聚化或者与其他Erb/Her受体异源二聚化。Epiregulin激活ERK1/2, and PI3K/Akt信号通路[1,2]。

原厂资料：

Source / Purification

Recombinant human Epiregulin (hEpiregulin) Val63-Leu108 (Accession #NP_001423) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Based on amino acid sequencing, greater than 50% of recombinant hEpiregulin has a Met on the amino-terminal Val63 (MVSIT) and has a calculated MW of 5,406. The remainder starts at Val63 (VSITK). DTT-reduced and non-reduced protein migrate as 5 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEpiregulin. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hEpiregulin was determined in a MCF 10A cell proliferation assay. The ED50 of each lot is between 0.10-0.85 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hEpiregulin.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hEpiregulin. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

Epiregulin is a member of the EGF family that is highly expressed in many cancer-specific cells, cells of the placenta and macrophages (1,2). Epiregulin induces keratinocyte and corneal epithelial cell proliferation, oocyte maturation and may function in blastocyst implantation during pregnancy (3-5). Active soluble epiregulin is produced by proteolytic processing of a transmembrane precursor. Epiregulin is the only EGF family member known to bind to both the ErbB1/HER1 and ErbB4/HER4 EGF receptors (6). Epiregulin binding induces receptor homodimerization or heterodimerization with other Erb/Her receptors. Epiregulin activates the ERK1/2, and PI3K/Akt pathways (1,3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.