Human Epigen

• 产品编号：CST-5493SC      品牌：CST       原厂货号：5493SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > EGF 表皮生长因子
• 应用分类：

描述：

Cell Signaling Technology的重组人源Epigen(hEpigen)丙氨酸24-丙氨酸104（编号#Q6UW88-1）经E.coli表达获得。重组人源Epigen在N-氨基末端有一个甲基化，分子量为9,087. DTT还原和非还原蛋白游离基团为8kDa多肽。因为分子内胱氨酸，非还原蛋白具有稍大的流动性。 预期在重组人源Epigen的N-氨基末端AVTVT由氨基酸测序验证。每6 μg还原和未还原的人源重组Epigen由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%。重组人源Epigen的生物活性由MCF-10A细胞增殖实验测定，每批产品的ED50在2-30 ng/ml之间。每1 μg hEpigen中所含内毒素少于0.01 ng。每1 μg hEpigen溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的经0.22 μm滤器过滤的溶液后冻干带有载体。经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物无载体。Epigen因因具有氨基酸同源性被定为EGF家族成员[1]。序列从小鼠角质化细胞克隆的mRNA获得。Epigen在正常人类乳腺组织和一些前列腺癌以及乳腺癌中表达[2]。与EGF相比，Epigen与EGF受体结合的亲和性要低。但Epigen在诱导细胞增殖方面显示更高活性[2]。Epigen在MDA-MB-468乳腺癌细胞中诱导多种反应。Epigen也对前列腺细胞和血管上皮细胞起效应。Epigen和ErbB1/Her1结合[2]，这种结合使得受体同源二聚化以及和其他Erb/Her受体的异源二聚化[2]。

原厂资料：

Source / Purification

Recombinant human Epigen (hEpigen) Ala24-Ala104 (Accession #Q6UW88-1) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hEpigen does not have a Met on the amino terminus and has a calculated MW of 9,087. DTT-reduced and non-reduced protein migrate as 8 kDa polypeptides with non-reduced protein having slightly greater mobility due to intramolecular cystines. The expected amino-terminal AVTVT of recombinant hEpigen was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEpigen. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hEpigen was determined in a MCF-10A cell proliferation assay. The ED50 of each lot is between 2-30 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hEpigen.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hEpigen. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

Epigen was identified as an EGF family member by amino acid sequence homology (1). The sequence was obtained from mouse keratinocyte mRNA. Epigen is expressed in normal human mammary tissue and in some prostate and breast carcinomas (2). Epigen binding to known EGF receptors is with lower affinity when compared to EGF. However, Epigen appears to be superior in inducing cell proliferation (2). Epigen induces responses in MDA-MB-468 breast carcinoma cells. Epigen also has effects on prostate cells and vascular endothelial cells (2). Epigen binds to ErbB1/Her1 (2). Binding causes receptor homodimerization and heterodimerization with other Erb/Her receptors (2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.