Diap1 Antibody

• 产品编号：CST-5486S      品牌：CST       原厂货号：5486S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Diap1 Antibody检测内源性Diap1总蛋白。通过人工合成人源Diap1蛋白羧基末端相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Formins蛋白是一个大型多域的actin成核/聚合作用蛋白质家族，其特征是它们的起催化作用FH2结构域。mammalian diaphanous-related formin (mDia/diap)亚家族包含mDia1/diap1、mDia2/diap3和mDia3/diap2，它们是Rho家族小GTPases的效应因子。在Rho作用下，mDia/diap蛋白涉及调节多种细胞功能包括细胞骨架动态、迁移、粘附、极性 和细胞形状（1,2）。GTP-bound Rho激活mDia1/diap1，这导致Rho-associated kinase (ROCK)依赖的应激纤维形成(3,4)。mDia1蛋白的Rho激活也已经显示调节serum response factor (SRF)依赖的转录(5)，并且也涉及到人类癌症表型例如ras介导转化、新陈代谢和细胞侵润（6）。Rho家族小GTPase cdc42蛋白激活的mDia3/diap2，其在哺乳动物细胞有丝分裂中调节微管到着丝粒的附着(7)。mDia2/diap3蛋白的Rho依赖的激活在胞质分裂中收缩环的装配起着重要作用(8,9)。

1. Schönichen, A. and Geyer, M. (2010) Biochim Biophys Acta 1803, 152-63.
2. Chesarone, M.A. et al. (2010) Nat Rev Mol Cell Biol 11, 62-74.
3. Watanabe, N. et al. (1999) Nat Cell Biol 1, 136-43.
4. Ishizaki, T. et al. (2001) Nat Cell Biol 3, 8-14.
5. Copeland, J.W. and Treisman, R. (2002) Mol Biol Cell 13, 4088-99.
6. Narumiya, S. et al. (2009) Cancer Metastasis Rev 28, 65-76.
7. Yasuda, S. et al. (2004) Nature 428, 767-71.
8. Watanabe, S. et al. (2010) Mol Biol Cell 21, 3193-204.
9. Watanabe, S. et al. (2008) Mol Biol Cell 19, 2328-38.

原厂资料：

Specificity / Sensitivity

Diap1 Antibody recognizes endogenous levels of total Diap1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Diap1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Formins are a family of large multidomain actin nucleation/polymerization proteins characterized by their catalytic FH2 domains. The mammalian diaphanous-related formin (mDia/diap) subfamily, including mDia1/diap1, mDia2/diap3 and mDia3/diap2, are effectors of Rho family small GTPases. In response to Rho, mDia/diap proteins are involved in the regulation of multiple cell functions including cytoskeletal dynamics, migration, adhesion, polarity and cell shape (reviewed in 1,2). 
 mDia1/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (3,4). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (5), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis and invasion (reviewed in 6). 
 mDia3/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (7). 
 Rho-dependent activation of mDia2/diap3 is important in assembly of the contractile ring during cytokinesis (8,9).

1. Schönichen, A. and Geyer, M. (2010) Biochim Biophys Acta 1803, 152-63.
2. Chesarone, M.A. et al. (2010) Nat Rev Mol Cell Biol 11, 62-74.
3. Watanabe, N. et al. (1999) Nat Cell Biol 1, 136-43.
4. Ishizaki, T. et al. (2001) Nat Cell Biol 3, 8-14.
5. Copeland, J.W. and Treisman, R. (2002) Mol Biol Cell 13, 4088-99.
6. Narumiya, S. et al. (2009) Cancer Metastasis Rev 28, 65-76.
7. Yasuda, S. et al. (2004) Nature 428, 767-71.
8. Watanabe, S. et al. (2010) Mol Biol Cell 21, 3193-204.
9. Watanabe, S. et al. (2008) Mol Biol Cell 19, 2328-38.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody