CHAF1A (D77D5) XP? Rabbit mAb

• 产品编号：CST-5480P      品牌：Cell Signaling Technology       原厂货号：5480P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CHAF1A (D77D5) XP® Rabbit mAb兔单抗能够检测内源性CHAF1A总蛋白水平。通过合成的人源CHAF1A蛋白Thr293位点周围相应的多肽片段去免疫动物从而制备出单克隆抗体。Chromatin assembly factor 1 (CAF-1)是一个组蛋白H3/H4伴侣复合物，在DNA复制和核苷酸删除修复时候它的功能是核小体的从头组装（de novo assembly）(1)。核小体的装配是两个过程，涉及到最初的一个组蛋白H3/H4四聚物沉淀到DNA上，随后一对组蛋白H2A/H2B二聚体的沉淀(1)。CAF-1与PCNA相互作用，并且定位到DNA复制和DNA修复点，在上述位置它的功能是去装配新合成的组蛋白H3/H4四聚体到复制中的DNA上(2-6)。组蛋白H2A/H2B 二聚体的装配需要附加的装配因子。CAF-1复合物由三种蛋白组成：CHAF1A (p150)、CHAF1B (p60) 和RBAP48 (p48 or RBBP4)。CHAF1A和CHAF1B蛋白是对CAF-1复合物的特定蛋白，而RBAP48是多种染色质修饰复合物的成分(1)。CHAF1A和CHAF1B表达水平与细胞内增殖有关联，并且两个蛋白在静止期的细胞中表达水平是有意思的下调(7)。

1. Adams, C.R. and Kamakaka, R.T. (1999) Curr Opin Genet Dev 9, 185-90.
2. Smith, S. and Stillman, B. (1989) Cell 58, 15-25.
3. Smith, S. and Stillman, B. (1991) EMBO J 10, 971-80.
4. Shibahara, K. and Stillman, B. (1999) Cell 96, 575-85.
5. Moggs, J.G. et al. (2000) Mol Cell Biol 20, 1206-18.
6. Gaillard, P.H. et al. (1996) Cell 86, 887-96.
7. Polo, S.E. et al. (2004) Cancer Res 64, 2371-81.

原厂资料：

Specificity / Sensitivity

CHAF1A (D77D5) XP® Rabbit mAb detects endogenous levels of total CHAF1A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr293 of human CHAF1A protein.

Background

Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7).

1. Adams, C.R. and Kamakaka, R.T. (1999) Curr Opin Genet Dev 9, 185-90.
2. Smith, S. and Stillman, B. (1989) Cell 58, 15-25.
3. Smith, S. and Stillman, B. (1991) EMBO J 10, 971-80.
4. Shibahara, K. and Stillman, B. (1999) Cell 96, 575-85.
5. Moggs, J.G. et al. (2000) Mol Cell Biol 20, 1206-18.
6. Gaillard, P.H. et al. (1996) Cell 86, 887-96.
7. Polo, S.E. et al. (2004) Cancer Res 64, 2371-81.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at -20°C. Do not aliquot the antibody.