CD2AP (A599) Antibody

• 产品编号：CST-5478S      品牌：CST       原厂货号：5478S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 CD2AP (A599) Antibody检测内源性CD2AP总蛋白。通过人工合成人源CD2AP蛋白Ala599位点周围相应的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。CD2AP蛋白是一个支架蛋白，其被认为膜蛋白联系到细胞骨架(1-3)。该蛋白在专门的细胞类型的紧密连接的形成中扮演重要角色，例如在肾小球的裂孔隔膜(4)。CD2AP蛋白也涉及T细胞表达的CD2与抗原呈递细胞之间的免疫突触(5)。研究证明 CD2AP蛋白和其它细胞支架蛋白之间的相互作用可能调节EGFR蛋白的内吞作用 (3)。

1. Kirsch, K.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 6211-6216.
2. Kirsch, K.H. et al. (2001) J. Biol. Chem. 276, 4957-4963.
3. Lynch, D.K. et al. (2003) J. Biol. Chem. 278, 21805-21813.
4. Kawachi, H. et al. (2006) Nephrology (Carlton) 11, 274-281.
5. Hutchings, N.J. et al. (2003) J. Biol. Chem. 278, 22396-22403.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

CD2AP (A599) Antibody recognizes endogenous levels of total CD2AP protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala599 of human CD2AP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (1-3). It plays a role in formation of tight junctions in specialized cell types such as the slit diaphragm in the kidney glomerulus (4). CD2AP is also involved in the immunological synapse between CD2 expressing T cells and antigen presenting cells (5). It has been shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (3). 

1. Kirsch, K.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 6211-6216.
2. Kirsch, K.H. et al. (2001) J. Biol. Chem. 276, 4957-4963.
3. Lynch, D.K. et al. (2003) J. Biol. Chem. 278, 21805-21813.
4. Kawachi, H. et al. (2006) Nephrology (Carlton) 11, 274-281.
5. Hutchings, N.J. et al. (2003) J. Biol. Chem. 278, 22396-22403.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody