BSP II Antibody

• 产品编号：CST-5468S      品牌：CST       原厂货号：5468S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BSP II抗体可以识别内源性BSP II蛋白。合成对应人BSP II蛋白氨基端及其邻近氨基酸残基序列的多肽免疫动物获得多克隆抗体。抗体由蛋白A和肽亲和层析纯化获得。骨唾液蛋白II（ BSP II）以分泌蛋白形式存在于骨骼细胞外基质中（1-2）。BSP II是小整联蛋白结合受体N-连接的糖蛋白（SIBLING）家族的一员它对细胞基质粘附和两个活性蛋白复合物间的连接时必须的（3）。BSP II灵活的结合能力使其能够迅速的与多种蛋白，钙和发育中矿化的骨结合（3-5）。BSP II在乳腺癌细胞羟基磷灰石微钙化的起始过程发挥重要作用，会导致骨转移（4,6,7）。最新证据显示下调BSP II可能会导致转移人乳腺癌细胞模型中转移病灶形成和骨转移的减少。

1. Papagerakis, P. et al. (2002) Bone 30, 377-85.
2. Bellahcène, A. et al. (1994) Cancer Res 54, 2823-6.
3. Fisher, L.W. et al. (2001) Biochem Biophys Res Commun 280, 460-5.
4. Castronovo, V. and Bellahcene, A. (1998) Int J Oncol 12, 305-8.
5. Adwan, H. et al. (2004) Cancer Gene Ther 11, 109-20.
6. Diel, I.J. et al. (1999) Clin Cancer Res 5, 3914-9.
7. Sharp, J.A. et al. (1999) Lab Invest 79, 869-77.

原厂资料：

Specificity / Sensitivity

The BSP II Antibody detects endogenous levels of total BSP II protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BSP II protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bone sialoprotein II (BSP II) exists as a secreted protein in extracellular matrix of the skeleton (1-2). BSP II is part of the small integrin binding ligand N-linked glycoprotein (SIBLING) family and its role is crucial for cell to matrix adhesion as well as between two active protein complexes (3). Flexible binding capacity of BSP II also allows it to rapidly associate with various proteins, calcium, and mineralized phases of developing bone (3-5). BSP II plays a central role in the initiation of hydroxyapatite microcalcification of breast cancer cells that contributes to the pathogenesis of bone metastasis (4,6,7). Recent evidence suggests that the downregulation of BSP II may lead to the reduced formation of metastatic lesions and bone metastasis in a human breast cancer model (5).

1. Papagerakis, P. et al. (2002) Bone 30, 377-85.
2. Bellahcène, A. et al. (1994) Cancer Res 54, 2823-6.
3. Fisher, L.W. et al. (2001) Biochem Biophys Res Commun 280, 460-5.
4. Castronovo, V. and Bellahcene, A. (1998) Int J Oncol 12, 305-8.
5. Adwan, H. et al. (2004) Cancer Gene Ther 11, 109-20.
6. Diel, I.J. et al. (1999) Clin Cancer Res 5, 3914-9.
7. Sharp, J.A. et al. (1999) Lab Invest 79, 869-77.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.