RagC (D31G9) XP? Rabbit mAb

• 产品编号：CST-5466S      品牌：CST       原厂货号：5466S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RagC (D31G9) XP™ Rabbit mAb兔单抗可以检测出内源性RagC总蛋白。单克隆抗体是采用人类RagC蛋白氨基末端周围残基相对应的合成肽段免疫动物生产的。mTORC1激酶复合物是调节细胞生长的一个关键组分（1,2）。活性由能量水平，生长因子和氨基酸调节（3,4）。最近的研究发现，RagA，RagB，RagC和RagD，这四个相关的GTPases与mTORC1中的raptor互作（1,2）。这些互作是mTORC1响应氨基酸信号 活化所必须的和有效的（1,2）。

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.

原厂资料：

Specificity / Sensitivity

RagC (D31G9) XP® Rabbit mAb detects endogenous levels of total RagC protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RagC protein.

Background

mTORC1 kinase complex is a critical component in regulating cell growth (1,2). Its activity is modulated by energy levels, growth factors and amino acids (3,4). Recent studies found that RagA, RagB, RagC and RagD, the four related GTPases, interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody