Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate)

• 产品编号：CST-5465S      品牌：CST       原厂货号：5465S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate)HRP偶联鼠单抗是一个高亲和性的抗体。ELISA中对大量磷酸化肽段的实验显示P-Tyr-100结合磷酸化酪氨酸，不受周围氨基酸序列影响。二维凝胶Western Blot分析过钒酸盐处理处理的细胞抽提物也显示P-Tyr-100与大量酪氨酸磷酸化的蛋白质发生相互作用。P-Tyr-100不与其它包含磷酸化丝氨酸和磷酸化苏氨酸的肽段发生相互作用。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）,该单克隆抗体用包含磷酸化酪氨酸的肽段免疫动物制备。CST公司生产的抗体与辣根过氧化物酶（HRP）的碳水化合物基团通过其氨基基团相结合。抗体预计可以和未结合HRP的Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411一样具有相同的交叉反应活性。赖氨酸磷酸化在细胞信号转导中扮演重要角色（1）。在肿瘤中，上调酪氨酸激酶活性可以通过产生不适当的增生和存活信号而导致恶性肿瘤和肿瘤形成（2）。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值（3,4）。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体作为非常新的高灵敏度研究工具，在酪氨酸磷酸化和高通量药物发现中监测酪氨酸激酶活性研究中的使用逐渐增加。

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

原厂资料：

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.

Description

This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody