Human _His6Fas Ligand/TNFSF6 (h_His6FasL)

• 产品编号：CST-5452SC      品牌：CST       原厂货号：5452SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TNF Family 肿瘤坏死因子
• 应用分类：

描述：

CST公司生产的人重组蛋白_His6FasL (h_His6FasL) Pro134-Leu281 (Accession #NP_000630)是从人的293细胞表达而来。重组的hFasL蛋白在N-末端有His6标签，蛋白分子量据推算为19,834Da。DTT-还原的蛋白和未还原的蛋白作为31-36kDa的多肽而迁移。在SDS-PAGE中较低的迁移率是由于糖基化的作用。6 μg 还原 (+) 和 非还原的(-) 重组h_His6FasL通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。h_His6FasL的生物活性是通过Jurkat细胞生存能力的实验来确定的。每个批次的ED50在1-5 ng/ml之间。每微克hFasL内毒素含量小于0.01 ng。

有载体：每微克h_His6FasL蛋白溶解在包含20 μg BSA的PBS（pH 7.2）的溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克h_His6FasL蛋白溶解在PBS（pH 7.2）溶液，并通过 0.22 μm 滤膜冻干。

FasL是TNF超家族蛋白中的一员，并且主要在激活的T细胞和NK细胞的表面表达(1)。FasL通过其诱导凋亡的能力来调控免疫反应。FasL的免疫调控能力在淋巴结病中被低估，主要表现在FasL或者Fas敲除小鼠会发生淋巴结病 ，FasL受体突变会导致人自体免疫淋巴增生综合症(1)。FasL是一个膜蛋白，能够被金属蛋白酶剪切形成可溶的三聚体形式(1,2)。可溶性的FasL仍然具有结合Fas的能力，然而其诱导凋亡的能力会消失(2)。Fas被FasL结合后，导致死亡诱导信号复合体(DISC)形成，并招募和激活caspase-8/caspase-10 (1,3)。激活的caspase-8/caspase-10随后激活“效应因子”caspases（caspase-3 和caspase-7）并剪切BID (1,3)。

原厂资料：

Source / Purification

Recombinant humanHis6 FasL (hHis6FasL) Pro134-Leu281 (Accession #NP_000630) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged hFasL has a calculated MW of 19,834. DTT-reduced and non-reduced protein migrate as 31-36 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis6FasL. All lots are greater than 98% pure.

Bioactivity

The bioactivity of hHis6FasL was determined in a Jurkat cell viability assay. The ED50 of each lot is between 1-5 ng/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis6FasL. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

FasL is a member of the TNF-superfamily family of proteins and is expressed primarily on the cell surface of activated T and NK cells (1). FasL regulates the immune response through its ability to induce apoptosis. The immunoregulatory role of FasL is underscored by lymphoadenopathy associated with FasL or Fas knockout mice and the fraction of autoimmune lympho-proliferative syndrome (ALPS) patients that have mutations in the FasL receptor, Fas (1). FasL is a membrane protein that can be cleaved into a soluble trimeric form by metalloproteinases (1,2). The soluble form of FasL retains the ability to bind to Fas, however, its ability to induce apoptosis is diminished (2). The ligation of Fas by FasL leads to the assembly of death-inducing signaling complex (DISC) and the recruitment and activation of caspase-8/caspase-10 (1,3). Active caspase-8/caspase-10 subsequently activates the “effector” caspases caspase-3 and caspase-7, and cleavage of BID (1,3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.