PEN2 Antibody

• 产品编号：CST-5451S      品牌：CST       原厂货号：5451S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PEN2 Antibody 兔多抗识别内源性的PEN2 总蛋白。该多克隆抗体由合成的人类PEN2蛋白Lys11位点附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。早老素增强子2（Presenilin Enhancer 2，PEN2）是一个小的膜整合的糖蛋白，它包含两个已识别的跨膜结构域。N-和C-末端域均在内质网的腔面（1）。PEN2，与早老素1、2，Nicastrin，和APH-1形成的蛋白复合物γ-分泌酶（2）。蛋白酶BACE催化APP处理的启示步骤，剪切和释放可溶性 APPβ（3）。剩下的膜结合APP被γ-分泌酶复合体裂解，导致β淀粉样肽的释放，形成淀粉样斑块。这些斑块是阿尔茨海默病的病理特征（2）。除了APP，γ-分泌酶还可裂解其它蛋白质，是早老素依赖的级联信号所需，包括Notch通路（4）。已发现PEN2是γ-分泌酶复合物的重要组成部分，基因下调可导致复合物数量的减少，导致γ-分泌酶活性降低（5）。

1. Sala Frigerio, C. et al. (2005) J Neurol 252, 1033-6.
2. Hansson, C.A. et al. (2004) J Biol Chem 279, 51654-60.
3. Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59.
4. St George-Hyslop, P. and Schmitt-Ulms, G. (2010) Nature 467, 36-7.
5. Steiner, H. et al. (2002) J Biol Chem 277, 39062-5.

原厂资料：

Specificity / Sensitivity

PEN2 Antibody detects endogenous levels of total PEN2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys11 of human PEN2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (1). PEN2, along with Presenilin 1, Presenilin 2, Nicastrin, and APH-1 form the protein complex γ-secretase (2). The proteinase BACE catalyses the initial step in APP processing by cleaving and releasing soluble APPβ (3). The remaining membrane bound APP is then cleaved by the γ-secretase complex, causing the release of amyloid β-peptide, the main constituent of amyloid plaques. These plaques are a hallmark of Alzheimer’s disease pathology (2). In addition to APP, the γ-secretase complex cleaves several other proteins and necessary presenilin-dependent signaling cascades, including the Notch pathway (4). It was found that PEN2 is an important part of the γ-secretase complex, and knocking it down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (5).

1. Sala Frigerio, C. et al. (2005) J Neurol 252, 1033-6.
2. Hansson, C.A. et al. (2004) J Biol Chem 279, 51654-60.
3. Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59.
4. St George-Hyslop, P. and Schmitt-Ulms, G. (2010) Nature 467, 36-7.
5. Steiner, H. et al. (2002) J Biol Chem 277, 39062-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody