Nanog (D73G4) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-5448S      品牌：CST       原厂货号：5448S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Nanog (D73G4) XP^® Rabbit mAb 兔单抗（偶联Alexa Fluor^® 647偶联）识别内源性的总nanog蛋白。此单克隆抗体由合成肽段免疫动物产生，该肽段与人nanog蛋白邻近氨基末端的氨基酸序列一致。Cell Signaling Technology公司抗体偶联Alexa Fluor^® 647荧光素染料，并经对人类细胞经直接流式细胞术和免疫荧光分析进行内部测试。抗体预期和未偶联Alexa Fluor^® 647的Nanog (D73G4) XP® Rabbit mAb兔 单抗#4903显示同样物种交叉反应。Nanog是一个含有同源结构域的转录因子，对于维持胚胎干细胞的多潜能性和自我更新非常重要（1）。Nanog的表达由包括Sox2和关键多潜能性调控因子Oct-4在内的调控网络调控（1）。近来对于体细胞重编程的研究进展主要通过整合包含nanog, Oct-4, Sox2, KLF4, c-Myc和 LIN28等转录因子病毒表达来实现 (2,3).

1. Kim, J. et al. (2008) Cell 132, 1049-61.
2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
3. Yu, J. et al. (2007) Science 318, 1917-20.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Nanog (D73G4) XP^® Rabbit mAb (Alexa Fluor^® 647 Conjugate) detects endogenous levels of total nanog protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nanog (D73G4) XP^® Rabbit mAb #4903.

Background

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

1. Kim, J. et al. (2008) Cell 132, 1049-61.
2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
3. Yu, J. et al. (2007) Science 318, 1917-20.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.