EpCAM (VU1D9) Mouse mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-5447S      品牌：CST       原厂货号：5447S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

EpCAM (VU1D9)鼠单抗（Alexa Fluor® 647标记）可以识别内源性总的EpCAM蛋白。用NCI-H69小细胞肺癌细胞免疫动物获得单克隆抗体。Cell Signaling Technology抗体与Alexa Fluor® 647荧光染料连接，经过在人细胞流式细胞术和免疫荧光分析的内部实验验证。抗体被认为与未标记的EpCAM (VU1D9) 鼠单抗#2929具有相同的反应原性。上皮细胞粘附和刺激分子（EpCAM/CD326）是一个跨膜糖蛋白，介导Ca2+非依赖同嗜性粘附到大部分上皮细胞的底外侧。EpCAM不表达在成体扁平上皮，但是在腺和扁平细胞瘤中表达（1）。作为被首次发现与肿瘤相关的抗原，长期以来EpCAM一直作为上皮和肿瘤组织的标记。由于它在癌症中高表达，EpCAM可以作为人上皮系实体瘤的治疗靶点（综述见2,3）。

1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
3. Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.

原厂资料：

Specificity / Sensitivity

EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total EpCAM protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated EpCAM (VU1D9) Mouse mAb #2929.

Background

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).

1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
3. Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.