SKAR α/β (D65E8) XP? Rabbit mAb

• 产品编号：CST-5439P      品牌：Cell Signaling Technology       原厂货号：5439P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SKAR α/β (D65E8) XP® Rabbit mAb兔单抗检测内源性SKAR总蛋白，包括α和β亚型。western blot显示在所有灵长类动物的细胞类型测试中该抗体可检测出一个不明来源的大约21 kDa条带。通过人工合成人源SKAR蛋白氨基末端相应的多肽片段去免疫动物从而制备单克隆抗体。p70 S6 kinase是一个丝裂原激活的丝/苏氨酸蛋白激酶，其位于PI3K的下游并且是rapamycin，FRAP/mTOR的靶标, FRAP/mTOR的下游分子。p70 S6 kinase对细胞生长和细胞周期进程是必须的(1,2)。SKAR蛋白最近发现是S6K1蛋白的一个底物。SKAR蛋白存在α 和β两个亚型，β亚型含有一个29氨基酸截断片段。SKAR蛋白磷酸化是丝裂原诱导的，且对rapamycin敏感。SKAR蛋白水平的减少导致细胞体积减小，进一步暗示SKAR蛋白在细胞生长控制中的作用(3)。

1. Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82.
2. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
3. Richardson, C.J. et al. (2004) Curr Biol 14, 1540-9.

原厂资料：

Specificity / Sensitivity

SKAR α/β (D65E8) XP® Rabbit mAb detects endogenous levels of total SKAR protein, α and β isoforms. By western blot, the antibody detects an approximately 21 kDa band of unknown origin in all primate cell types tested.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human SKAR.

Background

p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3).

1. Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82.
2. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
3. Richardson, C.J. et al. (2004) Curr Biol 14, 1540-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.