TERF2IP (D9H4) Rabbit mAb

• 产品编号：CST-5433S      品牌：CST       原厂货号：5433S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TERF2IP (D9H4) Rabbit mAb能够检测内源性TERF2IP总蛋白。该单克隆抗体是由合成的人源的针对TERF2IP蛋白的羧基末端肽段免疫动物生产的。端粒重复序列结合因子2相互作用蛋白(TERF2IP，也称为RAP1)是Shelterin复合物的一个组成部分，该复合物是一种多蛋白复合体，它能够结合和组织端粒至T-回路以阻止DNA双链损伤时其被细胞识别(1,2)。Shelterin复合物是由TERF2IP, TIN2和TPP2三种蛋白组成，除了能够招募复合体至端粒的三种DNA结合蛋白以外，TRF1和TRF2可以结合端粒末端的双链TTAGGG重复序列，而POT1能够结合端粒末端的单链TTAGGG重复序列(2)。同时，这些蛋白能够保护端粒和确保合理复制以及染色体末端处理。新近研究显示TERF2IP对于端粒长度的维持，端粒染色质的组织和端粒转录的调控是非必要的(3,4)。然而，TERF2IP为抑制同源定向修复(HDR)所必需，该修复能够带来不良的姐妹染色单体交换(3,4)。除了在端粒维持中的作用以外，TERF2IP亦被发现在细胞质中充当IκB激酶(IKK)适配子蛋白和调控NF-κB依赖的基因表达(5)。TERF2IP与IKKs组成一个蛋白复合体，它对IKKs的合理招募和NF-κB的p65亚基的激活至关重要。NF-κB功能亢进的乳腺癌细胞中已发现TERF2IP的水平升高，而TERF2IP敲除以后这些细胞对凋亡变得敏感，进一步表明了TERF2IP是一个潜在的肿瘤治疗靶点(5)。

1. Li, B. et al. (2000) Cell 101, 471-83.
2. de Lange, T. (2005) Genes Dev 19, 2100-10.
3. Sfeir, A. et al. (2010) Science 327, 1657-61.
4. Martinez, P. et al. (2010) Nat Cell Biol 12, 768-80.
5. Teo, H. et al. (2010) Nat Cell Biol 12, 758-67.

原厂资料：

Specificity / Sensitivity

TERF2IP (D9H4) Rabbit mAb detects endogenous levels of total TERF2IP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human TERF2IP protein.

Background

Telomeric repeat-binding factor 2-interacting protein (TERF2IP, also known as RAP1) is a component of the Shelterin Complex, a multi-protein complex that binds and organizes telomeres into T-loop structures to prevent them from being recognized by the cell as DNA double stranded breaks (1,2). The Shelterin Complex is composed of TERF2IP, TIN2 and TPP2 proteins, in addition to three DNA binding proteins that function to recruit the complex to telomeres: TRF1 and TRF2 bind double-stranded TTAGGG repeats found at telomeres, while the POT1 protein binds single-stranded TTAGGG repeats found at the very end of the telomeres (2). Together, these proteins function to protect telomeres and ensure proper replication and processing of chromosome ends. Recent studies have shown that TERF2IP is dispensable for maintenance of telomere length, organization of telomeric chromatin, and regulation of telomeric transcription (3,4). However, TERF2IP is required for inhibition of homology-directed repair (HDR), which can create undesirable telomeric sister chromatid exchange (3,4). In addition to its role in telomere maintenance, TERF2IP is also found in the cytoplasm, where it functions as an IκB kinase (IKK) adaptor protein and regulates NF-κB-dependent gene expression (5). TERF2IP forms a complex with IKKs and is critical for proper recruitment of IKKs to and activation of the p65 subunit of NF-κB. Elevated levels of TERF2IP have been found in breast cancer cells with NF-κB hyperactivity, and knockdown of TERF2IP sensitizes these cells to apoptosis, further identifying TERF2IP as a potential cancer therapeutic target (5). 

1. Li, B. et al. (2000) Cell 101, 471-83.
2. de Lange, T. (2005) Genes Dev 19, 2100-10.
3. Sfeir, A. et al. (2010) Science 327, 1657-61.
4. Martinez, P. et al. (2010) Nat Cell Biol 12, 768-80.
5. Teo, H. et al. (2010) Nat Cell Biol 12, 758-67.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.