RACK1 (D59D5) Rabbit mAb

• 产品编号：CST-5432S      品牌：CST       原厂货号：5432S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RACK1 (D59D5) Rabbit mAb兔单抗识别内源性RACK1总蛋白。通过人工合成人源RACK1蛋白中间序列相应的多肽片段去免疫动物从而制备出单克隆抗体。高度保守receptor for activated C kinase 1 (RACK1)与异源三聚体βG蛋白的β亚基同源，该蛋白起初通过它与活化的PKCβII和其它传统的PKC亚基的结合被鉴定(1)。RACK1蛋白是一个支架蛋白，它能招募PKC和大范围的特异亚细胞定位的其它蛋白质，从而促进多种蛋白质复合物的形成去诱导和整合不同信号通路(2)。该蛋白的一个例子是PKC依赖的JNK激活的提高(3)。RACK1蛋白也停留在真核核糖体，认为 RACK1蛋白参与信号复合物的装配，该复合物也可调节翻译(4)。RACK1蛋白结合到Src蛋白的SH2区域，并且在PKC激活后，通过Src蛋白使RACK1蛋白在Tyr228位点发生磷酸化(5)。

1. Ron, D. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 839-843.
2. Lopez-Bergami , P. et al. (2005) Mol. Cell 19, 309-320.
3. McCahill, A. et al. (2002) Mol. Pharmacol. 62, 1261-1273.
4. Nilsson, J. et al. (2004) EMBO Rep. 5, 1137-1141.
5. Chang, B. Y. et al. (2002) Oncogene 21, 7619-7629.

原厂资料：

Specificity / Sensitivity

RACK1 (D59D5) Rabbit mAb recognizes endogenous levels of total RACK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RACK1 protein.

Background

The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5).

1. Ron, D. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 839-843.
2. Lopez-Bergami , P. et al. (2005) Mol. Cell 19, 309-320.
3. McCahill, A. et al. (2002) Mol. Pharmacol. 62, 1261-1273.
4. Nilsson, J. et al. (2004) EMBO Rep. 5, 1137-1141.
5. Chang, B. Y. et al. (2002) Oncogene 21, 7619-7629.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody