MSH6 (D60G2) XP? Rabbit mAb

• 产品编号：CST-5424P      品牌：Cell Signaling Technology       原厂货号：5424P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MSH6 (D60G2) XP® Rabbit mAb能够检测内源性MSH6总蛋白。该单克隆抗体是由合成的人源的针对MSH6蛋白羧基末端的肽段免疫动物而生产的。DNA错配修复系统(MMR)能够修复复制后DNA，抑制非相同DNA序列间的重组，诱导伴随某些DNA损伤类型的检验点和凋亡反应(1)。MSH2 (MutS 同源蛋白2)和MSH6组成的hMutS-α二聚体是错配修复过程必不可少的部分。hMutS-α是BRCA1相关监视蛋白复合体(BRSC)的一部分，该复合体还包括BRCA1, MLH1, ATM, BLM, PMS蛋白和Rad50-Mre11-NBS1复合体(2)。在大部分遗传性非息肉性大肠癌（Lynch综合征）中已发现MSH6和其它MMR蛋白的突变，该疾病是西方国家最常见的遗传性结肠癌(3)。研究发现恶性胶质瘤中MSH6发生突变以响应替莫唑胺治疗，并可以促进替莫唑胺抵抗(4)。

1. O'Brien, V. and Brown, R. (2006) Carcinogenesis 27, 682-92.
2. Wang, Y. et al. (2000) Genes Dev 14, 927-39.
3. Plotz, G. et al. (2006) J Mol Histol 37, 271-83.
4. Yip, S. et al. (2009) Clin Cancer Res 15, 4622-9.

原厂资料：

Specificity / Sensitivity

MSH6 (D60G2) XP® Rabbit mAb detects endogenous levels of total MSH6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MSH6.

Background

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).

1. O'Brien, V. and Brown, R. (2006) Carcinogenesis 27, 682-92.
2. Wang, Y. et al. (2000) Genes Dev 14, 927-39.
3. Plotz, G. et al. (2006) J Mol Histol 37, 271-83.
4. Yip, S. et al. (2009) Clin Cancer Res 15, 4622-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody