LIN28B Antibody (Mouse Preferred)

• 产品编号：CST-5422S      品牌：CST       原厂货号：5422S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LIN28B抗体（小鼠优选）识别小鼠内源性的总LIN28B蛋白。合成与小鼠LIN28B羧基末端的氨基酸残基序列一致的肽段，免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。LIN28A 和LIN28B是一类发育过程中保守的RNA结合调控蛋白，它抑制let-7家族的miRNAs的处理加工和成熟过程[1,2]。let-7 miRNAs已经显示抑制ras、myc和Hmga2等癌基因[3]。近来LIN28A 和 LIN28B被显示在人类主要肿瘤和肿瘤细胞系中上调表达。它们的过表达直接与let-7 miRNAs下调相关[4]。LIN28基因被报道原生殖细胞发育和精原细胞恶性肿瘤相关[5]。LIN28B的等位基因变异与人类青春期周期调控有关[6]。过表达的LIN28B协同Oct-4, Sox2, 和Nanog可使人成纤维细胞重编程为多潜能ES样细胞[7]。

1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
3. Cho, W.C. (2007) Mol Cancer 6, 60.
4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
5. West, J.A. et al. (2009) Nature 460, 909-13.
6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
7. Yu, J. et al. (2007) Science 318, 1917-20.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

LIN28B Antibody (Mouse Preferred) detects endogenous levels of total mouse LIN28B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse LIN28B. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
3. Cho, W.C. (2007) Mol Cancer 6, 60.
4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
5. West, J.A. et al. (2009) Nature 460, 909-13.
6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
7. Yu, J. et al. (2007) Science 318, 1917-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.