Bub1b (D32E8) Rabbit mAb

• 产品编号：CST-5421S      品牌：CST       原厂货号：5421S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bub1b (D32E8)兔源单克隆抗体能够检测内源性的Bub1b总蛋白。该单克隆抗体是由合成的人源的针对Bub1b蛋白lys370附近区域氨基酸肽段免疫动物而生产的。有丝分类检验点复合体(MCC)包括Bub1，Bub1b，Bub3，Mad2和Cdc20，它们控制染色体分离并监视着丝粒-微管相互作用(1)。有丝分裂中，MCC抑制泛素连接酶APC/C的活性，从而防止染色体未配对的细胞过早地进入分裂后期(2)。在多种人类恶性肿瘤如造血癌、结肠癌、肺癌和乳腺癌中存在Bub1b 和Bub1激酶的突变(3)。在花叶杂色非整倍体综合征和过早染色单体分离综合征中都发现了Bub1b等位基因的突变(4)。小鼠生殖细胞Bub1b敲除后胚胎致死，动物杂合子显示出基因组不稳定性，早老表型，癌症易感性增加(5)。Bub3能够结合Bub1和Bub1b，促进其在着丝粒的招募(6)，并参与功能性的微管-着丝粒相互作用(7)。

1. Fukagawa, T. (2008) Front Biosci 13, 2705-13.
2. Chen, R.H. (2007) J Biomed Sci 14, 475-9.
3. Dai, W. et al. (2004) Cancer Res 64, 440-5.
4. Kops, G.J. et al. (2005) Nat Rev Cancer 5, 773-85.
5. Baker, D.J. et al. (2004) Nat Genet 36, 744-9.
6. Taylor, S.S. et al. (1998) J Cell Biol 142, 1-11.
7. Logarinho, E. et al. (2008) Mol Biol Cell 19, 1798-813.

原厂资料：

Specificity / Sensitivity

Bub1b (D32E8) Rabbit mAb detects endogenous levels of total Bub1b protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Lys370 of human Bub1b. 

Background

The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase (2). Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6), and is required for functional microtubule-kinetochore interactions (7). 

1. Fukagawa, T. (2008) Front Biosci 13, 2705-13.
2. Chen, R.H. (2007) J Biomed Sci 14, 475-9.
3. Dai, W. et al. (2004) Cancer Res 64, 440-5.
4. Kops, G.J. et al. (2005) Nat Rev Cancer 5, 773-85.
5. Baker, D.J. et al. (2004) Nat Genet 36, 744-9.
6. Taylor, S.S. et al. (1998) J Cell Biol 142, 1-11.
7. Logarinho, E. et al. (2008) Mol Biol Cell 19, 1798-813.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody