GCNF Antibody

• 产品编号：CST-5417S      品牌：CST       原厂货号：5417S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GCNF抗体识别内源性的总GCNF蛋白。合成与人源GCNF349蛋白的邻近Aka349氨基酸残基序列一致的肽段，免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。GCNF（生殖细胞核因子），又称为NR6A1（核受体亚家族6族A成员），是属于核受体基因超家族的一个孤独核受体（1）。发育时期的神经系统和成体卵巢和精巢内生殖细胞的特定发育时期能够表达GCNF，并且在配子发生，神经系统发育和原肠胚的一般的胚胎发育过程中发挥功能（1,2）。小鼠中GCNF失活会导致臀部发育畸形，中脑发育损伤，神经管闭合不全甚至胚胎死亡（3）。经证实GCNF是OCT-4和鱼精蛋白的抑制因子（4,5），并且在胚胎发生和精子生产过程中起着调控基因表达的重要作用（2,6）。

1. Lan, Z.J. et al. (2009) Biol Reprod 80, 905-12.
2. Lei, W. et al. (1997) J Mol Endocrinol 18, 167-76.
3. Chung, A.C. et al. (2001) Mol Cell Biol 21, 663-77.
4. Akamatsu, W. et al. (2009) J Neurosci 29, 2113-24.
5. Hummelke, G.C. and Cooney, A.J. (2004) Mol Reprod Dev 68, 394-407.
6. Lan, Z.J. et al. (2002) J Biol Chem 277, 50660-7.

原厂资料：

Specificity / Sensitivity

GCNF Antibody detects endogenous levels of total GCNF protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic polypeptide corresponding to residues surrounding Ala349 of human GCNF. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

GCNF (Germ Cell Nuclear Factor), also known as NR6A1 (Nuclear Receptor Subfamily 6 Group A member), is an orphan member of the nuclear receptor gene superfamily (1). It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation (1,2). Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death (3). GCNF has been shown to be a repressor of OCT-4 and of the protamine genes (4,5) and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis (2,6).

1. Lan, Z.J. et al. (2009) Biol Reprod 80, 905-12.
2. Lei, W. et al. (1997) J Mol Endocrinol 18, 167-76.
3. Chung, A.C. et al. (2001) Mol Cell Biol 21, 663-77.
4. Akamatsu, W. et al. (2009) J Neurosci 29, 2113-24.
5. Hummelke, G.C. and Cooney, A.J. (2004) Mol Reprod Dev 68, 394-407.
6. Lan, Z.J. et al. (2002) J Biol Chem 277, 50660-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.