NME1/NDKA (D18F10) Rabbit mAb

• 产品编号：CST-5389S      品牌：CST       原厂货号：5389S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NME1/NDKA (D18F10) Rabbit mAb用于检测内源性NME1/NDKA总蛋白水平。抗体可与NME2/NDKB蛋白交互反应。该单抗通过用对应于人源NME1/NDKA蛋白Pro96位点周围残基序列的合成肽免疫动物制备而得。NDK/NME/NM23激酶家族（由NME基因家族编码）至少包括八种不同的蛋白，蛋白有不同的细胞定位（1）。该族激酶可以抑制多种肿瘤细胞的转移（2）。所有NDK/NME/NM23都有二磷酸核苷酸激酶活性，可以催化二磷酸核苷酸成三磷酸核苷酸，这可以调节多种细胞的生理反应（3）。NDK至少有四种主要的生物作用已经得到充分的研究，包括蛋白相互作用（4-6），GTP-结合蛋白功能的调控（7-9），DNA结合活性（10，11）和组蛋白依赖性的蛋白磷酸转移酶活性（12）。NDK/NME蛋白在多种细胞反应中发挥调控作用，包括发育，分化，增殖，内吞和凋亡（13）。NDKA (NME1/NM23-H1)由于在肿瘤新生和转移抑制作用，已经得到了充分地研究（14）。NDKA (NM23-H1)和NDKB (NM23-H2) 由相邻和 NME1和NME2 基因调控，并且序列有90%的相似性。NDKA/NM23-H1上的两个残基（Ser122和Ser144）可以被AMPKα1磷酸化，但只有Ser122上的磷酸化可以决定NDKA是否将ATP转移给AMPKα1.这会调控AMPKα1的活性向ACC1靠拢（它是脂肪酸代谢过程中的一个关键调控因子）（15）。黑色素瘤细胞中NDKB/NM23-H2在Ser122(S122P)位点的突变会改变其磷酸根转移活性（16）。

1. Lacombe, M.L. et al. (2000) J Bioenerg Biomembr 32, 247-58.
2. Tee, Y.T. et al. (2006) Taiwan J Obstet Gynecol 45, 107-13.
3. Ishikawa, N. et al. (2003) J Bioenerg Biomembr 35, 7-18.
4. Paravicini, G. et al. (1996) Biochem Biophys Res Commun 227, 82-7.
5. Reymond, A. et al. (1999) Oncogene 18, 7244-52.
6. Subramanian, C. et al. (2001) Nat Med 7, 350-5.
7. Zhu, J. et al. (1999) Proc Natl Acad Sci USA 96, 14911-8.
8. Otsuki, Y. et al. (2001) Proc Natl Acad Sci USA 98, 4385-90.
9. Palacios, F. et al. (2002) Nat Cell Biol 4, 929-36.
10. Fan, Z. et al. (2003) Cell 112, 659-72.
11. Postel, E.H. (2003) J Bioenerg Biomembr 35, 31-40.
12. Wagner, P.D. and Vu, N.D. (2000) Biochem J 346 Pt 3, 623-30.
13. Kimura, N. et al. (2000) J Bioenerg Biomembr 32, 309-15.
14. Steeg, P.S. (2004) J Natl Cancer Inst 96, E4.
15. Crawford, R.M. et al. (2006) Mol Cell Biol 26, 5921-31.
16. Schaertl, S. et al. (1999) J Biol Chem 274, 20159-64.

原厂资料：

Specificity / Sensitivity

NME1/NDKA (D18F10) Rabbit mAb detects endogenous levels of total NME1/NDKA protein. This antibody is predicted to cross-react with NME2/NDKB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro96 of human NME1/NDKA protein.

Background

The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including protein-protein interactions (4-6), regulation of GTP-binding protein function (7-9), DNA-associated activities (10,11), and histidine-dependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied (14). NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKα1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKα1. This regulates AMPKα1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16).

1. Lacombe, M.L. et al. (2000) J Bioenerg Biomembr 32, 247-58.
2. Tee, Y.T. et al. (2006) Taiwan J Obstet Gynecol 45, 107-13.
3. Ishikawa, N. et al. (2003) J Bioenerg Biomembr 35, 7-18.
4. Paravicini, G. et al. (1996) Biochem Biophys Res Commun 227, 82-7.
5. Reymond, A. et al. (1999) Oncogene 18, 7244-52.
6. Subramanian, C. et al. (2001) Nat Med 7, 350-5.
7. Zhu, J. et al. (1999) Proc Natl Acad Sci USA 96, 14911-8.
8. Otsuki, Y. et al. (2001) Proc Natl Acad Sci USA 98, 4385-90.
9. Palacios, F. et al. (2002) Nat Cell Biol 4, 929-36.
10. Fan, Z. et al. (2003) Cell 112, 659-72.
11. Postel, E.H. (2003) J Bioenerg Biomembr 35, 31-40.
12. Wagner, P.D. and Vu, N.D. (2000) Biochem J 346 Pt 3, 623-30.
13. Kimura, N. et al. (2000) J Bioenerg Biomembr 32, 309-15.
14. Steeg, P.S. (2004) J Natl Cancer Inst 96, E4.
15. Crawford, R.M. et al. (2006) Mol Cell Biol 26, 5921-31.
16. Schaertl, S. et al. (1999) J Biol Chem 274, 20159-64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.