RXRα Antibody

• 产品编号：CST-5388S      品牌：CST       原厂货号：5388S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RXRα Antibody识别内源性水平的总 RXRα蛋白。RXRα Antibody不与RXRβ或RXRγ中的任何一个发生交叉反应。该多克隆抗体通过用合成肽免疫动物制备，该合成肽是人RXRα 蛋白靠近氨基末端的残基。抗体由蛋白A和肽亲和层析纯化。人视黄醇X受体（RXRs）由三种不同的基因（RXRα，RXRβ，RXRγ）编码，并与具有高亲和力的维生素A衍生物，9-顺-视黄酸选择性结合。 RXRs是II型核激素受体，主要位于核室，与非依赖性配体结合。核RXR与核激素受体亚族蛋白形成异二聚体，包括甲状腺激素受体、维甲酸受体、维生素D受体、过氧化物酶体增殖物活化受体、肝脏X受体、法尼酯X受体（1）。由于RXR与多个核激素受体形成异源二聚体，通过与靶基因启动子/增强区域的顺式作用元件结合，在众多激素信号转导通路的转录调控中发挥核心作用（2）。基因敲除小鼠RXRα基因会导致胚胎心肌发育不全使胚胎致死，这也证明 RXRα对体内视黄醇信号的重要性(5,6)。生化证据表明，RXRα转录活性是通过 Ras-Raf-MAPK信号级联在翻译后调节的。MAPK依赖性RXRα的磷酸化，直接去除RXRα与核受体共激活因子关联的能力（7）。

1. Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
2. Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
3. Mangelsdorf, D.J. et al. (1990) Nature 345, 224-9.
4. Dollé, P. et al. (1994) Mech Dev 45, 91-104.
5. Kastner, P. et al. (1994) Cell 78, 987-1003.
6. Sucov, H.M. et al. (1994) Genes Dev 8, 1007-18.
7. Macoritto, M. et al. (2008) J Biol Chem 283, 4943-56.

原厂资料：

Specificity / Sensitivity

RXRα Antibody recognizes endogenous levels of total RXRα protein. RXRα Antibody does not cross-react with either RXRβ or RXRγ.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RXRα protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).Retinoid X receptor α (RXRα) is the founding RXR family member and is predominantly expressed in liver, kidney, epidermis, intestine and a variety of tissues (2-4). Knockout of the murine rxrα gene results in embryonic lethality tentatively due to myocardial hypoplasia, which demonstrates the importance of RXRα to retinoid signaling in vivo (5,6). Biochemical evidence suggests that RXRα transcriptional activity is post-translationally regulated through the Ras-Raf-MAPK signaling cascade. MAPK-dependent phosphorylation of RXRα directly abrogates the ability of RXRα to associate with nuclear receptor coactivators (7).

1. Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
2. Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
3. Mangelsdorf, D.J. et al. (1990) Nature 345, 224-9.
4. Dollé, P. et al. (1994) Mech Dev 45, 91-104.
5. Kastner, P. et al. (1994) Cell 78, 987-1003.
6. Sucov, H.M. et al. (1994) Genes Dev 8, 1007-18.
7. Macoritto, M. et al. (2008) J Biol Chem 283, 4943-56.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody.