Mouse Oncostatin M (mOSM)

• 产品编号：CST-5371SC      品牌：CST       原厂货号：5371SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它细胞因子和生长因子
• 应用分类：

描述：

CST公司在大肠杆菌中生产重组鼠源蛋白OSM (mOSM) Ala24-Arg206 (Accession #NP_001013383)。重组的 mOSM蛋白在N-末端没有Met，其分子量据推算为20,750Da。DTT-还原和未还原的蛋白迁移时是作为一个21kDa 的多肽而转移。重组mOSM蛋白的N-末端ANRGC的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) mOSM通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hOSM的生物活性是通过NIH/3T3 MEF WT细胞的细胞增殖实验确定的。每个批次的ED50在0.10-0.40 ng/ml之间。

有载体: 每微克mOSM蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mOSM蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

制瘤素M (OSM)是IL-6细胞因子家族成员，它主要是由激活的T细胞核巨噬细胞产生(1,2)。OSM诱导纤维原细胞的增殖，抑制肿瘤细胞增殖并在免疫调控中发挥了重要作用(2,3)。小鼠OSM结合到OSM受体β (OSMRβ)并与普遍的IL-6家族受体亚单元gp130形成异源性复合体(4)。相反，人OSM结合到两个不同的受体复合体上,OSMRβ/gp130和 LIFRβ/gp130复合体(4)。人源的OSM结合到两个受体上的能力解释了人OSM和LIF的重叠和不同。OSM诱导Jak2, Stat3 和 Erk1/2 信号通路的激活(5)。

原厂资料：

Source / Purification

Recombinant mouse OSM (mOSM) Ala24-Arg206 (Accession #NP_001013383) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mOSM does not have Met on the amino terminus and has a calculated MW of 20,750. DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides. The expected amino-terminal ANRGC of recombinant mOSM was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mOSM. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mOSM was determined in a NIH/3T3 MEF WT cell proliferation assay. The ED50 of each lot is between 0.10-0.40 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mOSM.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mOSM. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Mouse OSM binds to the OSM receptor β (OSMRβ) which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). In contrast, human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. OSM induces the activation the JAK2, STAT3, and ERK1/2 pathways (5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.