LAP2α (3A3) Mouse mAb

• 产品编号：CST-5369S      品牌：CST       原厂货号：5369S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LAP2α (3A3) Mouse mAb 鼠单抗能够检测内源性水平LAP2α总蛋白。该单克隆抗体是采用合成的人源LAP2α蛋白C末端相对应的肽段免疫动物而制备的。核纤层蛋白与核纤层蛋白相关蛋白是核纤层的主要组成部分，研究发现其存在于内核膜和周围染色质之间。这些蛋白质在保持核结构、染色质组织、DNA复制、细胞周期调控和细胞凋亡方面发挥着重要作用（1-3）。核纤层蛋白是V型核纤层蛋白中间丝，进一步可分为A型和B型。 A型核纤层蛋白（包括核纤层蛋白A和较小的核纤层蛋白Ç剪接变异体）主要在终末分化细胞中表达，而B型核纤层蛋白（核纤层蛋白B1，核纤层蛋白B2）由不同的基因编码表达。在细胞凋亡过程中，核纤层蛋白作为细胞被拆除的部分，被半胱天冬酶裂解（4-6）。一定数量的核纤层蛋白相关蛋白作用于核纤层，包括核纤层蛋B受体、LAP1、LAP2、emerin、MAN1、otefin和YA。研究已经描述了 lamina 相关多肽2（LAP2，也被称为胸腺生成素或TMPO）的若干异构体，在人体中α、β和γ异构体最为丰富（7-10）。结构上类似的LAP2β和LAP2γ是Ⅱ型整合膜蛋白。LAP2α具有独特的羧基末端，缺乏一个跨膜区，导致LAP2α的定位贯穿细胞核，在那里它可以与核纤层蛋白A/C作用（10）。LAP2α也被认为有助于视网膜母细胞瘤蛋白（RB）的核锚定，并能够控制细胞周期进程（11）。LAP2α也是半胱天冬酶裂解靶点，这可能有助于细胞凋亡过程中的染色质结构的变化（12）。

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
3. Holmer, L. and Worman, H.J. (2001) Cell Mol Life Sci 58, 1741-7.
4. Lazebnik, Y.A. et al. (1995) Proc Natl Acad Sci USA 92, 9042-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
7. Furukawa, K. et al. (1995) EMBO J 14, 1626-36.
8. Foisner, R. and Gerace, L. (1993) Cell 73, 1267-79.
9. Harris, C.A. et al. (1994) Proc Natl Acad Sci USA 91, 6283-7.
10. Dechat, T. et al. (2000) J Cell Sci 113 Pt 19, 3473-84.
11. Markiewicz, E. et al. (2002) Mol Biol Cell 13, 4401-13.
12. Gotzmann, J. et al. (2000) J Cell Sci 113 Pt 21, 3769-80.

原厂资料：

Specificity / Sensitivity

LAP2α (3A3) Mouse mAb detects endogenous levels of total LAP2α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LAP2α protein.

Background

Lamins and lamin associated proteins are the major components of nuclear lamina found between the inner nuclear membrane and the peripheral chromatin. These proteins play important roles in maintaining nuclear structure, chromatin organization, DNA replication, cell cycle regulation, and apoptosis (1-3). Lamins are type V intermediate filaments that are further classified into type A and type B lamin proteins. Type A lamins (including lamin A and the smaller lamin C splice variant) are predominately expressed in terminally differentiated cells, whereas type B lamins (lamin B1, lamin B2) are encoded by distinct genes and are expressed constitutively. Cleavage of lamins by caspases occurs during apoptosis as part of the disassembly of the cell (4-6). A number of lamina-associated proteins contribute to the nuclear lamina and include the lamin B receptor, LAP1, LAP2, emerin, MAN1, otefin, and YA. Several isoforms of lamina-associated polypeptide 2 (LAP2, also known as thymopoietin or TMPO) have been described, with the α, β, and γ isoforms most abundant in humans (7-10). Structurally similar LAP2β and LAP2γ are type II integral membrane proteins. LAP2α has a unique carboxy-terminus that lacks a transmembrane region and results in localization of LAP2α throughout the nucleus where it can associate with lamin A/C (10). LAP2α is also thought to contribute to the nuclear anchorage of retinoblastoma protein (Rb) and control cell cycle progression (11). LAP2α is also targeted for cleavage by caspases, which may contribute to changes in chromatin structure during apoptosis (12). 

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
3. Holmer, L. and Worman, H.J. (2001) Cell Mol Life Sci 58, 1741-7.
4. Lazebnik, Y.A. et al. (1995) Proc Natl Acad Sci USA 92, 9042-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
7. Furukawa, K. et al. (1995) EMBO J 14, 1626-36.
8. Foisner, R. and Gerace, L. (1993) Cell 73, 1267-79.
9. Harris, C.A. et al. (1994) Proc Natl Acad Sci USA 91, 6283-7.
10. Dechat, T. et al. (2000) J Cell Sci 113 Pt 19, 3473-84.
11. Markiewicz, E. et al. (2002) Mol Biol Cell 13, 4401-13.
12. Gotzmann, J. et al. (2000) J Cell Sci 113 Pt 21, 3769-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody