Human Oncostatin M (hOSM)

• 产品编号：CST-5367SF      品牌：CST       原厂货号：5367SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它细胞因子和生长因子
• 应用分类：

描述：

CST公司在人293细胞中生产重组人蛋白Oncostatin M (hOSM) Ala26-Arg221 (Accession #NP_065391)。基于氨基酸测序结果，85%以上的重组hOSM蛋白在Aa26 (AAIGS)位点起始，据推算分子量为19,249Da。其余的蛋白在Ile28 (IGSCS)位点起始。DTT-还原和未还原蛋白作为32kDa多肽而转移。在SDS-PAGE中的低迁移率是由于糖基化的原因。6 μg 还原 (+) 和 非还原的(-) hOSM通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hOSM的生物活性是通过TF-1细胞的细胞增殖实验确定的。每个批次的ED50在50-500 pg/ml之间。内毒素含量:<0.01 ng /1 μg hOSM。

有载体: 每微克hOSM蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hOSM蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

制瘤素M (OSM)是IL-6细胞因子家族成员，它主要是由激活的T细胞核巨噬细胞产生(1,2)。OSM诱导纤维原细胞的增殖，抑制肿瘤细胞增殖并在免疫调控中发挥了重要作用(2,3)。小鼠OSM结合到OSM受体β (OSMRβ)并与普遍的IL-6家族受体亚单元gp130形成异源性复合体(4)。相反，人OSM结合到两个不同的受体复合体上,OSMRβ/gp130和 LIFRβ/gp130复合体(4)。人源的OSM结合到两个受体上的能力解释了人OSM和LIF的重叠和不同。OSM诱导Jak2, Stat3 和 Erk1/2 信号通路的激活(5)。

原厂资料：

Source / Purification

Recombinant human Oncostatin M (hOSM) Ala26-Arg221 (Accession #NP_065391) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Based on amino acid sequencing, greater than 85% of recombinant hOSM starts at Aa26 (AAIGS) and has a calculated MW of 19,249. The remainder starts at Ile28 (IGSCS). DTT-reduced and non-reduced protein migrate as 32 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hOSM. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hOSM was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 50-500 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hOSM.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hOSM. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. In contrast, Mouse OSM binds to the OSM receptor β (OSMRβ) which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). OSM induces the activation the Jak2, Stat3, and Erk1/2 pathways (5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.