ENPP1 (D37B7) Rabbit mAb

• 产品编号：CST-5342S      品牌：CST       原厂货号：5342S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ENPP1 (D37B7) Rabbit mAb用于检测内源性ENPP1总蛋白水平。该单抗通过用对应于人源ENPP1蛋白Leu520位点周围残基序列的合成肽免疫动物制备而得。核苷酸内焦磷酸酶/磷酸二酯酶1（ENPP1）是二型单次跨膜蛋白，主要在质膜上ATP的水解中发挥作用。ENPP1在细胞基膜和侧膜表面的定位依赖于羧基化的二亮氨酸信号（1）。ENPP1在骨矿质化和软组织钙化中发挥作用（2-5）。ENPP1基因的突变会导致广义的婴儿期动脉钙化（GACI）和先天性婴儿动脉钙化（IIAC）（6，7）。ENPP1 会抑制胰岛素受体功能，该酶的过表达会导致胰岛素抵抗和血糖不耐受（8,9）。ENPP1的基因变异跟肥胖和2型糖尿病相关（10-12）。

1. Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
2. Okawa, A. et al. (1998) Nat Genet 19, 271-3.
3. Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
4. Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
5. Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
6. Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
7. Ruf, N. et al. (2005) Hum Mutat 25, 98.
8. Dong, H. et al. (2005) Diabetes 54, 367-72.
9. Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
10. Meyre, D. et al. (2005) Nat Genet 37, 863-7.
11. Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
12. Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

ENPP1 (D37B7) Rabbit mAb detects endogenous levels of total ENPP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu520 of human ENPP1 protein.

Background

Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. Targeting of ENPP1 to the basolateral cell surface relies on the presence of a carboxy-terminal di-leucine-based signal (1). ENPP1 plays important roles in bone mineralization and soft tissue calcification (2-5). Mutations in the corresponding ENPP1 gene cause generalized arterial calcification in infancy (GACI) and idiopathic infantile arterial calcification (IIAC) (6,7). ENPP1 inhibits insulin receptor function and overexpression of this enzyme causes insulin resistance and glucose intolerance in mice (8,9). Genetic variants of ENPP1 have been associated with obesity and type 2 diabetes (10-12).

1. Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
2. Okawa, A. et al. (1998) Nat Genet 19, 271-3.
3. Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
4. Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
5. Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
6. Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
7. Ruf, N. et al. (2005) Hum Mutat 25, 98.
8. Dong, H. et al. (2005) Diabetes 54, 367-72.
9. Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
10. Meyre, D. et al. (2005) Nat Genet 37, 863-7.
11. Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
12. Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.