Mouse Epidermal Growth Factor (mEGF)

• 产品编号：CST-5331SC      品牌：CST       原厂货号：5331SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > EGF 表皮生长因子
• 应用分类：

描述：

在Cell Signaling Technology公司，在大肠杆菌中表达重组鼠源蛋白EGF (mEGF) Asn977-Arg1029 (Accession #NP_034243) 。重组的mFGF氨基末端含有甲硫氨酸，计算得到的分子量为6045.DTT-还原和非还原状态下蛋白迁移状态与6KDa多肽相同。重组mFGF氨基端序列MNSYP通过氨基酸测序确认。6 μg还原和未还原的mEGF由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%重组mEGF使用NIH/3T3细胞扩增实验检验其生物活性。每个批次的ED50值都在40-250ng/ml。1ug mEGF所含内毒素少于0.01ng。带载体：每1 μ mEGF溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的溶液经0.22 μm滤器过滤的溶液后冻干。无载体：经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物。EGF存在于上皮细胞，成纤维细胞和许多其他的细胞类型中（1,2）。低分子量可溶型EGF由一个分子量超过130,000的跨膜前体蛋白经蛋白水解生成（1,2）。可溶型和跨膜EGF都是有活性的（2）。EGF可以诱导多种细胞包括肿瘤来源的细胞增殖，分化和存活（1-3）。EGF家族和ErbB/HER EGF受体家族有许多成员。EGF结合ErbB1/HER1诱导同源二聚化或与其他ErbB/HER成员的异源二聚化（1）。EGF，EGF家族成员，EGF受体和它们的信号通路涉及到多种癌症，可以作为介入治疗的靶点（1,2）。

原厂资料：

Source / Purification

Recombinant mouse EGF (mEGF) Asn977-Arg1029 (Accession #NP_034243) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mEGF has a Met on the amino terminus and has a calculated MW of 6045. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSYP of recombinant mEGF was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mEGF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mEGF was determined in an NIH/3T3 cell proliferation assay. The ED50 of each lot is between 40-250 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mEGF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg mEGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.