JMJD2A (C37E5) Rabbit mAb

• 产品编号：CST-5328S      品牌：CST       原厂货号：5328S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

JMJD2A (C37E5) Rabbit mAb兔单抗能够检测内源性JMJD2A总蛋白水平。该抗体不与JMJD2B、JMJD2C或JMJD2D蛋白发生交叉反应。通过合成的与人源JMJD2A蛋白Val444位点周围相应的多肽片段去免疫动物从而制备出此单克隆抗体。组蛋白赖氨酸的甲基化水平对于基因组的活化和非活化区域的形成是主要决定因素，并且在发育期间对于基因组的正确进程起着关键作用(1,2)。包含蛋白质的Jumonji C (JmjC)区域代表最大的潜在组蛋白去乙酰化酶蛋白(3)。JmjC区域通过氧化反应能催化单、双和三甲基化的赖氨酸残基的去乙甲基化，这种氧化反应需要铁离子和α-酮戊二酸(3)。基于同源性，人源和小鼠都包含至少30种这样蛋白质，这能够被分为7个不同的家族(3)。JMJD2 (Jumonji domain-containing protein 2)家族也称为JHDM3 (JmjC domain-containing histone demethylation protein 3)家族，包含四个成员：JMJD2A/JHDM3A、JMJD2B/JHDM3B、JMJD2C/JHDM3C和JMJD2D/JHDM3D。除了JmJC结构域之外，这些蛋白质也包含JmJN、PHD和Tudor结构域，而后者基因证明能结合到甲基化的histone H3蛋白Lys4和Lys9 位点，以及甲基化的histone H4蛋白Lys20位点上(4,5)。研究证明JMJD2蛋白可以使双核三甲基化的histone H3蛋白Lys9和Lys36位点上去甲基化，并且具有转录的激活和抑制因子的功能(6-11)。JMJD2A、JMJD2C 和JMJD2D作为在前列腺肿瘤细胞中雄激素受体的共激活因子(7)。与此相反，JMJD2A也与Rb和N-CoR共抑制因子复合物有关联，并且对于靶基因的转录抑制是必须的(8,9)。在着丝点周围异染色质上JMJD2B蛋白抑制histone H3蛋白Lys9位点三甲基化(10)。JMJD2C也称为GASC1，它在鳞状细胞癌和转移性肺癌中扩增，并且JMJD2C蛋白表达的抑制减少了细胞扩增(11,12)。JMJD2C蛋白也被鉴定作为Oct-4的下游靶蛋白，并且在胚胎干细胞中对于自我更新的调节起到重要作用(13)。

1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
4. Chen, Z. et al. (2007) Proc Natl Acad Sci USA 104, 10818-23.
5. Lee, J. et al. (2008) Nat Struct Mol Biol 15, 109-11.
6. Whetstine, J.R. et al. (2006) Cell 125, 467-81.
7. Shin, S. and Janknecht, R. (2007) Biochem Biophys Res Commun 359, 742-6.
8. Gray, S.G. et al. (2005) J Biol Chem 280, 28507-18.
9. Zhang, D. et al. (2005) Mol Cell Biol 25, 6404-14.
10. Fodor, B.D. et al. (2006) Genes Dev 20, 1557-62.
11. Cloos, P.A. et al. (2006) Nature 442, 307-11.
12. Italiano, A. et al. (2006) Cancer Genet Cytogenet 167, 122-30.
13. Loh, Y.H. et al. (2007) Genes Dev 21, 2545-57.

原厂资料：

Specificity / Sensitivity

JMJD2A (C37E5) Rabbit mAb recognizes endogenous levels of total JMJD2A protein. This antibody does not cross-react with JMJD2B, JMJD2C, or JMJD2D.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val444 of human JMJD2A protein.

Background

The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The JMJD2 (Jumonji domain-containing protein 2) family, also known as JHDM3 (JmjC domain-containing histone demethylation protein 3) family, contains four members: JMJD2A/JHDM3A, JMJD2B/JHDM3B, JMJD2C/JHDM3C and JMJD2D/JHDM3D. In addition to the JmJC domain, these proteins also contain JmJN, PHD and Tudor domains, the latter of which has been shown to bind to methylated histone H3 at Lys4 and Lys9, and methylated histone H4 at Lys20 (4,5). JMJD2 proteins have been shown to demethylate di- and tri-methyl histone H3 at Lys9 and Lys36, and function as both activators and repressors of transcription (6-11). JMJD2A, JMJD2C and JMJD2D function as coactivators of the androgen receptor in prostate tumor cells (7). In contrast, JMJD2A also associates with Rb and N-CoR corepressor complexes and is necessary for transcriptional repression of target genes (8,9). JMJD2B antagonizes histone H3 Lys9 tri-methylation at pericentric heterochromatin (10). JMJD2C, also known as GASC1, is amplified in squamous cell carcinomas and metastatic lung carcinoma, and inhibition of JMJD2C expression decreases cell proliferation (11,12). JMJD2C has also been identified as a downstream target of Oct-4 and is critical for the regulation of self-renewal in embryonic stem cells (13).

1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
4. Chen, Z. et al. (2007) Proc Natl Acad Sci USA 104, 10818-23.
5. Lee, J. et al. (2008) Nat Struct Mol Biol 15, 109-11.
6. Whetstine, J.R. et al. (2006) Cell 125, 467-81.
7. Shin, S. and Janknecht, R. (2007) Biochem Biophys Res Commun 359, 742-6.
8. Gray, S.G. et al. (2005) J Biol Chem 280, 28507-18.
9. Zhang, D. et al. (2005) Mol Cell Biol 25, 6404-14.
10. Fodor, B.D. et al. (2006) Genes Dev 20, 1557-62.
11. Cloos, P.A. et al. (2006) Nature 442, 307-11.
12. Italiano, A. et al. (2006) Cancer Genet Cytogenet 167, 122-30.
13. Loh, Y.H. et al. (2007) Genes Dev 21, 2545-57.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.