Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb

• 产品编号：CST-5327S      品牌：CST       原厂货号：5327S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb鼠单抗能够检测仅在Lys9位点双甲基化或三甲基化的内源性histone H3的蛋白水平。该抗体也显示与Lys9位点单甲基化histone H3发生弱交叉反应。该抗体不与Lys4、Lys27、Lys36或Lys79位点甲基化的histone H3蛋白发生交叉反应。通过人工合成仅在lys9位点三甲基化histone H3蛋白氨基端相应的多肽片段去免疫动物从而制备出单克隆抗体。核小体是由四种组蛋白(H2A、H2B、H3和H4)组成，它是染色质的主要构成模块。起初被认为作为一个DNA包装的静态支架，现在则显示组蛋白是动态蛋白，经历多种翻译后修饰的形式，包括乙酰化、磷酸化、甲基化和泛素化(1)。组蛋白甲基化对于该基因组的活化和未活化区域的形成有着主要决定作用，并且在发育期间对该基因组的正确规划起着关键作用(2,3)。histones H3 (Arg2、17、26)和H4 (Arg3)的精氨酸甲基化促进转录调控以及通过蛋白质精氨酸甲基转移酶(PRMTs)家族的介导，包括共激活因子PRMT1和CARM1 (PRMT4) (4)。相反，多种多样的组蛋白赖氨酸甲基转移酶已经被鉴定，除了这个之外其它的都包含一个保守的催化SET区域，这个起初被鉴定在Drosophila Su(var)3-9、zeste增强子和Trithorax蛋白。赖氨酸甲基化主要发生在histones H3 (Lys4、9、27、36、79)和H4 (Lys20)，并且已经涉及到转录激活和沉默(4)。这些赖氨酸残基的甲基化协调染色质修饰酶的招募包括methyl-lysine结合模块例如chromodomains (HP1, PRC1)、PHD fingers (BPTF, ING2)、tudor domains (53BP1)和WD-40 domains (WDR5) (5-8)。组蛋白例如PADI4、LSD1、JMJD1、JMJD2和JHDM1的发现已经显示甲基化是一个可逆的表遗传标记物(9)。

1. Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
2. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
3. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
4. Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70.
5. Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26.
6. Shi, X. et al. (2006) Nature 442, 96-9.
7. Wysocka, J. et al. (2006) Nature 442, 86-90.
8. Wysocka, J. et al. (2005) Cell 121, 859-72.
9. Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.

原厂资料：

Specificity / Sensitivity

Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb detects endogenous levels of histone H3 when di- or tri-methylated on Lys9. The antibody also shows slight cross-reactivity with histone H3 when mono-methylated on Lys9. The antibody does not cross-react with methylated histone H3 Lys4, Lys27, Lys36 or Lys79.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is tri-methylated.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).

1. Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
2. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
3. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
4. Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70.
5. Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26.
6. Shi, X. et al. (2006) Nature 442, 96-9.
7. Wysocka, J. et al. (2006) Nature 442, 86-90.
8. Wysocka, J. et al. (2005) Cell 121, 859-72.
9. Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.