Mouse Vascular Endothelial Growth Factor-120 (mVEGF120 )

• 产品编号：CST-5314LF      品牌：CST       原厂货号：5314LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > VEGF血管内皮生长因子
• 应用分类：

描述：

CST公司生产的鼠源重组蛋白VEGF120 (mVEGF120) Ala27-Arg146 (Accession #NP_001020428)是从人源293细胞表达而来。重组的mVEGF120蛋白没有标签，未糖基化的蛋白分子量据推算为14,071Da。DTT-还原蛋白由于糖基化的存在而作为一个20-26kDa的蛋白转移,因此在SDS-PAGE中有较低的迁移率。未还原的蛋白由于有半胱氨酸连接形成同源二聚体而作为32-37 kDa 蛋白迁移。重组hVEGF120蛋白的N-末端APTTE的序列通过测序得到。重组蛋白mVEGF120的生物活性是通过HUVEC细胞的增殖实验确定的。每个批次的ED50值在1-5 ng/ml之间。内毒素含量：小于0.01 ng /1 μg mVEGF120。

有载体: 每微克mVEGF120蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mVEGF120蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

VEGF120 ,是VEGF-A基因的一种剪切形式,其存在于鼠科中，与人的VEGF121形式及其相似(1,2)。VEGF120能够通过内皮细胞、巨噬细胞、T细胞和其它的一些细胞类型产生。VEGF120参与到了血管再生、血管内皮细胞生存、生长、迁移和血管渗透(1)。VEGF120的表达受到组织缺氧、炎症因子和肿瘤组织产生的致癌基因的诱导(1,2)。结合到VEGFR1和VEGFR2 受体酪氨酸激酶(1)。VEGF121 结合到 VEGFR1和VEGFR2导致了信号通路的激活，如PI3K/AKT, P38 MAPK和 FAK (1)。在很多癌症中，VEGF在肿瘤血管再生中发挥了重要的作用(2)。

原厂资料：

Source / Purification

Recombinant mouse VEGF120 (mVEGF120) Ala27-Arg146 (Accession #NP_001020428) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mVEGF120 contains no "tags" and the nonglycosylated protein has a calculated MW of 14,071. DTT-reduced protein migrates as a 20-26 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 32-37 kDa protein. The expected amino-terminal APTTE of recombinant hVEGF120 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF120. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mVEGF120 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-5 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg mVEGF120.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mVEGF120. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

VEGF120 , a splice variant of the VEGF-A gene, is the murine counterpart to human VEGF121 (1,2). VEGF120 is produced by endothelial cells, macrophages, T cells, and others. VEGF120 is involved in angiogenesis, vascular permeability, vascular endothelial cell survival, growth, and migration (1). VEGF120 expression is induced by hypoxia, inflammatory cytokines, and through oncogene products in tumors (1,2). VEGF120 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF120 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/Akt, p38, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.