RBPSUH (D10A4) XP? Rabbit mAb

• 产品编号：CST-5313P      品牌：Cell Signaling Technology       原厂货号：5313P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RBPSUH (D10A4) XP^® Rabbit mAb可以识别内源性的总RBPSUH蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人RBPSUH蛋白邻近110位谷氨酰胺氨基酸残基序列一致。RBPSUH(Recombining Binding Protein, SUppressor of Hairless),也称为RBP-J或CSL，是canonical Notch 信号通路调控的转录复合物的DNA结合组分。在缺少Notch激活的情况下，RBPSUH通过与一个含有组蛋白去乙酰基酶的共抑制复合物结合发挥抑制靶基因表达的作用。激活Notch受体后，Notch细胞内结构域（NICD）转位到细胞核，并与RBPSUH结合。此时共抑制复合物会被置换为一个转录激活复合物包括Mastermind-like (MAML)蛋白和组蛋白乙酰化酶p300，引起Notch靶基因的转录激活（1-3）。RBPSUH也是Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2)的DNA结合伴侣，EBNA2是一个对潜伏病毒转录和EBV-感染的B细胞永生化非常重要的蛋白（4,5）。

1. Ehebauer, M. et al. (2006) Sci STKE 2006, cm7.
2. Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46.
3. Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33.
4. Henkel, T. et al. (1994) Science 265, 92-5.
5. Strobl, L.J. et al. (1997) Immunobiology 198, 299-306.

原厂资料：

Specificity / Sensitivity

RBPSUH (D10A4) XP^® Rabbit mAb recognizes endogenous levels of total RBPSUH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln110 of human RBPSUH protein.

Background

RBPSUH (Recombining Binding Protein, SUppressor of Hairless), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes (1-3). RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells (4,5).

1. Ehebauer, M. et al. (2006) Sci STKE 2006, cm7.
2. Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46.
3. Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33.
4. Henkel, T. et al. (1994) Science 265, 92-5.
5. Strobl, L.J. et al. (1997) Immunobiology 198, 299-306.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.