Human RANKL/TRANCE/TNFSF11 (hRANKL)

• 产品编号：CST-5312SF      品牌：CST       原厂货号：5312SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TNF Family 肿瘤坏死因子
• 应用分类：

描述：

CST公司利用重组的人 RANKL (hRANKL) Gly63-Asp244 (Accession #NP_143026)在人的293细胞中表达。重组的hRANKL蛋白在N-末端没有标签，其分子量据推算为20,484Da。DTT-还原和未还原的蛋白迁移时是作为一个30-35kDa 的多肽而转移。其在SDS-PAGE胶中的双重迁移性质与低迁移速率是由于糖基化。重组hRANKL蛋白的N-末端序列 GSQHI通过测序得到。6 μg 还原 (+) 和 非还原的(-) hRANKL通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hRANKL的生物活性是通过测定其在Raw 264.7细胞中对活性TRAP诱导能力确定。每个批次的ED50 在1.5-5 ng/ml之间。内毒素含量:<0.01 ng /1 μg hRANKL.

有载体: 每微克hRANKL蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hRANKL蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

RANKL，也叫做TRANCE或OPGL, TNF家族配体中的一员。T 细胞、乳腺上皮细胞核内皮细胞能够产生RANKL(1)。RANKL作为II类跨膜蛋白或经胞外蛋白酶剪切形成可溶形式，如TACE, ADAM10 和基质金属蛋白酶(1)。可变剪切也导致了可溶性RANKL的产生 (1)。RANKL信号被osteoprotegerin所对抗, 并作为可溶性诱捕受体(2)。RANKL在乳腺发育和树枝状细胞存活中发挥了重要的作用，并且是破骨细胞分化和存活所必须(3-6)。研究表明在小鼠和人中RANKL的缺失都与不正常的骨密度增加和淋巴组织形成缺陷有关(5,6)。

原厂资料：

Source / Purification

Recombinant human RANKL (hRANKL) Gly63-Asp244 (Accession #NP_143026) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hRANKL contains no "tags" and the nonglycosylated protein has a calculated MW of 20,484. DTT-reduced and non-reduced protein migrate as 30-35 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal GSQHI of recombinant hRANKL was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hRANKL. All lots are greater than 98% pure.

Bioactivity

The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 of each lot is between 1.5-5 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hRANKL.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hRANKL. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 5% sucrose.

Background

RANKL, also known as TRANCE or OPGL, is a member of the TNF superfamily of ligands. T cells, mammary epithelial cells, and endothelial cells can produce RANKL (1). RANKL is expressed as a type II transmembrane protein or cleaved into a soluble form by extracellular proteases, such as TACE, ADAM10, and matrix metalloproteases (1). Alternative splicing also results in the production of soluble RANKL (1). RANKL signaling is antagonized by osteoprotegerin, which functions as a soluble decoy receptor (2). RANKL plays key roles in mammary gland development and dendritic cell survival and is required for osteoclast differentiation and survival (3-6). Research studies have shown that RANKL deficiencies in both mice and humans are associated with abnormally increased bone density and defects in lymphoid organogenesis (5,6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.