Synapsin-1 (D12G5) XP? Rabbit mAb

• 产品编号：CST-5297P      品牌：Cell Signaling Technology       原厂货号：5297P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Synapsin-1 (D12G5) XP® Rabbit mAb 兔单抗识别内源性的synapsin 总蛋白。用来制作抗原的肽段序列与人synapsin-1a和synapsin-1b 100%保守。该单克隆抗体由合成的 人类synapsin-1蛋白Gln483位点附近肽段免疫动物而制成。Synapsins是一组至少包括五个相关成员（Synapsins IA，IB，IIA，IIB，和IIIa）的蛋白，在脑内丰富，在调节神经递质的释放中必不可少（1,2）。所有的synapsins都包含一个短的氨基末端结构域，它高度保守，可被PKA或钙调蛋白激酶I磷酸化（1）。synapsin氨基末端域Ser9的磷酸化会抑制其与磷脂的结合，将其从突触囊泡上分解出来（2）。

1. Greengard, P. (1987) Mol Neurobiol 1, 81-119.
2. Hosaka, M. et al. (1999) Neuron 24, 377-87.

原厂资料：

Specificity / Sensitivity

Synapsin-1 (D12G5) XP® Rabbit mAb detects endogenous levels of total synapsin protein. The antigen is 100% conserved between human synapsin-1a and synapsin-1b.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln483 of human synapsin-1 protein.

Background

Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).

1. Greengard, P. (1987) Mol Neurobiol 1, 81-119.
2. Hosaka, M. et al. (1999) Neuron 24, 377-87.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody