Phospho-CTDSPL2 (Ser104) Antibody

• 产品编号：CST-5294S      品牌：CST       原厂货号：5294S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CTDSPL2 (Ser104) Antibody检测仅在Ser104位点磷酸化的内源性CTDSPL2总蛋白。通过合成的与人源CTDSPL2蛋白Ser104位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

CTD small phosphatase-like protein 2 (CTDSPL2, HSPC129)是一个假设RNA-polymerase II carboxy-terminal domain (CTD)磷酸酶(1)，这属于CTD磷酸酶的一个小亚家族(2)。RNA polymerase II的CTD包含多重的Y-S-P-T-S-P-S重复序列，该序列是在转录循环期间被磷酸化(3,4)。一般而言，在RNA合成的数个步骤和在转录后修饰期间，CTD磷酸酶调节RNA-polymerase II的可逆CTD磷酸化作用(4-6)。CTDSPL2蛋白有数个结构性和功能性类似与其它CTD磷酸酶，包括FCP1、SCP1、DULLARD和UBLCP1 (1,2)。

Cell Signaling Technology (CST)使用PhosphoScan® 和LC-MS/MS平台发现了CTDSPL2蛋白在Ser104位点的磷酸化(7)。在选择癌细胞系和肿瘤中也发现了该位点(8)。

1. Qian, H. et al. (2007) Mol Cell Biochem 303, 183-8.
2. Kim, Y. et al. (2007) Proc Natl Acad Sci USA 104, 6596-601.
3. Corden, J.L. et al. (1985) Proc Natl Acad Sci USA 82, 7934-8.
4. Ahn, S.H. et al. (2004) Mol Cell 13, 67-76.
5. Dahmus, M.E. (1996) J Biol Chem 271, 19009-12.
6. Goodrich, J.A. and Tjian, R. (1994) Cell 77, 145-56.
7. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
8. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.

原厂资料：

Specificity / Sensitivity

Phospho-CTDSPL2 (Ser104) Antibody detects endogenous levels of CTDSPL2 only when phosphorylated at Ser104.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human CTDSPL2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CTD small phosphatase-like protein 2 (CTDSPL2, HSPC129) is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase (1) that belongs to a small subfamily of CTD phosphatases (2). The CTD of RNA polymerase II contains multiple Y-S-P-T-S-P-S repeats that are phosphorylated during the transcription cycle (3,4). In general, CTD phosphatases regulate the reversible CTD phosphorylation state of RNA-polymerase II at several stages of RNA synthesis and during post-transcriptional modification (4-6). CTDSPL2 has several structural and functional similarities to other CTD phosphatases, including FCP1, SCP1, DULLARD, and UBLCP1 (1,2).

Phosphorylation of CTDSPL2 at Ser104 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (7). The site was independently found in select carcinoma cell lines and in tumors (8).

1. Qian, H. et al. (2007) Mol Cell Biochem 303, 183-8.
2. Kim, Y. et al. (2007) Proc Natl Acad Sci USA 104, 6596-601.
3. Corden, J.L. et al. (1985) Proc Natl Acad Sci USA 82, 7934-8.
4. Ahn, S.H. et al. (2004) Mol Cell 13, 67-76.
5. Dahmus, M.E. (1996) J Biol Chem 271, 19009-12.
6. Goodrich, J.A. and Tjian, R. (1994) Cell 77, 145-56.
7. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
8. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.