Mouse Interleukin-1α (mIL-1α)

• 产品编号：CST-5273SC      品牌：CST       原厂货号：5273SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-1 Family 白细胞介素-1超家族
• 应用分类：

描述：

CST公司在大肠杆菌中生产重组鼠源蛋白IL-1α (mIL-1α) Ser115-Ser270 (Accession #NP_034684)。重组的mIL-1α 蛋白在N-末端没有Met，其分子量据推算为17,990Da。DTT-还原和未还原的蛋白迁移时是作为一个18kDa 的多肽而转移。重组mIL-1α蛋白的N-末端SAPYT的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) mIL-1α通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白mIL-1α的生物活性是通过诱导3T3 MEFs WT细胞产生IL-6的能力实验确定的。每个批次的ED50在3-8 pg/ml之间。内毒素含量:<0.01 ng /1 μg mIL-1α。

有载体: 每微克mIL-1α蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIL-1α蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-1α是促炎症因子，主要由激活的单核细胞、淋巴细胞核上皮细胞产生(1)。IL-1α作为有活性的前体而合成，但是经过细胞质的蛋白酶剪切形成成熟形式(1,2)。通常来讲，前体和成熟形式的IL-1α主要是保留在细胞内而不是组成型的分泌(1,2)。通过IL-1α的信号由IL-1α结合到IL-1的附属蛋白(IL-1-AcP)，然后此复合体结合到IL-1RI (1,2)。信号通路的发生要通过激活MAP 激酶和NF-κB信号通路(1,2)。IL-1α也能结合到缺少胞内信号域的IL-RII上，并因此作为高亲和性的诱捕受体。IL-1α活性被抑制是通过IL-1R拮抗剂(IL-1Ra)，它结合IL-1R1但是不发生信号。在小鼠中。IL-1α是主要的病毒诱导的炎症应答的转化者(3)。

原厂资料：

Source / Purification

Recombinant mouse IL-1α (mIL-1α) Ser115-Ser270 (Accession #NP_034684) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mIL-1α does not have a Met on the amino terminus and has a calculated MW of 17,990. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminus SAPYT of recombinant mIL-1α was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-1α. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mIL-1α was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 3-8 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-1α.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NFκB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. Inhibition of IL-1α activity is through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.