Fetuin A (F180) Antibody

• 产品编号：CST-5258S      品牌：CST       原厂货号：5258S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fetuin A (F180) Antibody用于检测内源性的Fetuin A总蛋白水平。多克隆抗体是通过合成对应于人fetuin A蛋白Phe180位点肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。胎球蛋白A在肝脏中产生并且分泌到血浆中去，选择性地在骨基质中浓缩。它由前体的形式合成，然后经剪切形成两个由二硫键相连的肽链。它是一个全身性的抑制软组织钙化的蛋白（1）。在肾脏透析的患者中，胎球蛋白A水平的降低跟伴随着心血管死亡的高风险（2，3）。胎球蛋白A可以抑制由胰岛素诱导的胰岛素受体酪氨酸激酶及胰岛素受体底物1的酪氨酸磷酸化活性（4）。胎球蛋白A缺陷小鼠表现出高的胰岛素敏感性，人类血清中高水平的胎球蛋白A跟胰岛素抵抗相关（5.6）。

1. Schafer, C. et al. (2003) J Clin Invest 112, 357-66.
2. Westenfeld, R. et al. (2007) Trends Cardiovasc Med 17, 124-8.
3. Westenfeld, R. et al. (2009) J Am Soc Nephrol 20, 1264-74.
4. Auberger, P. et al. (1989) Cell 58, 631-40.
5. Mathews, S.T. et al. (2002) Diabetes 51, 2450-8.
6. Stefan, N. et al. (2006) Diabetes Care 29, 853-7.

原厂资料：

Specificity / Sensitivity

Fetuin A (F180) Antibody detects endogenous levels of total fetuin A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe180 of human fetuin A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Fetuin A is produced by liver and secreted to plasma, and selectively concentrated in bone matrix. It is synthesized as a precursor and processed to generate 2 chains that are held together by a disulfide bond. It is a systemically acting calcification inhibitor (1). Reduced level of fetuin A in serum is associated with increased cardiovascular mortality in dialysis patients (2,3). Fetuin A can inhibit insulin-induced tyrosine phosphorylation of the insulin receptor tyrosine kinase and insulin receptor substrate-1 (4). Fetuin A deficient mice show high insulin sensitivity and high levels of serum fetuin A are associated with insulin resistance in humans (5,6).

1. Schafer, C. et al. (2003) J Clin Invest 112, 357-66.
2. Westenfeld, R. et al. (2007) Trends Cardiovasc Med 17, 124-8.
3. Westenfeld, R. et al. (2009) J Am Soc Nephrol 20, 1264-74.
4. Auberger, P. et al. (1989) Cell 58, 631-40.
5. Mathews, S.T. et al. (2002) Diabetes 51, 2450-8.
6. Stefan, N. et al. (2006) Diabetes Care 29, 853-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody