Anti-mouse IgG (H+L) (DyLight® 800 Conjugate) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.
Source / Purification
This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.
Description
Anti-mouse IgG (H+L) was conjugated to DyLight® 800 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 777 nm and peak fluorescence emission is 794 nm.
Applications
This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western™.
Directions for Use
The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications. Fluorescent western blotting: 1:15000 In-Cell Western: 1:2000
Background
Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence..
注意事项：
Storage: Supplied in 100 mM PBS, pH 7.2, containing 1% BSA
and 0.02% sodium azide. Store at 4ºC. Protect from light. Do
not freeze.
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