Phospho-TCTP (Ser46) Antibody

• 产品编号：CST-5251S      品牌：CST       原厂货号：5251S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-TCTP (Ser46) Antibody检测仅在Ser46位点磷酸化的内源性TCTP蛋白。在一些细胞类型中，该抗体可能识别一个80-100 kDa大小的未知来源的蛋白。通过人工合成人源TCTP蛋白Ser46位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Translationally controlled tumor protein (TCTP/p23/HRF)是一个广泛表达的，并且高度保守的蛋白，该蛋白涉及不同细胞内进程，包括作为一个在慢性变态反应性疾病中组胺释放因子的角色(1)。TCTP以一个细胞周期依赖的方式结合微管蛋白，并且与细胞分裂纺锤体有关联。此外，TCTP与肌动蛋白细胞骨架相互作用去调节细胞形态 (3)。在有丝分裂中，TCTP蛋白通过PLK激酶使Ser46位点磷酸化，这减少微管的稳定性(4,5)。TCTP蛋白有小GTPase Rheb相互作用，很可能作为一个GEF，因此激活TORC1通路，从而控制细胞生长和增殖(6,7)。TCTP已经证实是涉及凋亡和细胞应激(8-11)。在培养的细胞中，TCTP蛋白表达的减少能引起癌症逆转 (12)。

1. MacDonald, S.M. et al. (1995) Science 269, 688-90.
2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
5. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
7. Hsu, Y.C. et al. (2007) Nature 445, 785-8.
8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
12. Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.

原厂资料：

Specificity / Sensitivity

Phospho-TCTP (Ser46) Antibody recognizes endogenous levels TCTP protein only when phosphorylated at Ser46. In some cell types the antibody may recognize an 80-100 kDa protein of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, including a role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF and thus activating the TORC1 pathway, controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction of TCTP expression can cause tumor reversion (12).

1. MacDonald, S.M. et al. (1995) Science 269, 688-90.
2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
5. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
7. Hsu, Y.C. et al. (2007) Nature 445, 785-8.
8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
12. Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.