Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb

• 产品编号：CST-5243S      品牌：CST       原厂货号：5243S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb识别内源性的含Ser-phospho-Thr-Pro基序的肽段。此抗体与磷酸化苏氨酸-脯氨酸基序有很弱的交叉反应。该单克隆抗体用SpTP肽段库免疫动物制备。Polo-like kinases (PLKs)是丝氨酸/苏氨酸激酶，在细胞周期中扮演重要角色。哺乳动物细胞中至少存在四种PLK：PLK1、PLK2、PLK3和PLK4（1）。PLK具有高度保守的氨基末端激酶结构域和一个相对不保守的羧基末端结构域Polo-box域（PBD）。在四种PLK中，PLK1是描述得最清楚的（2）。PLK1作为有丝分裂事件的关键调节者而发挥作用，它可以磷酸化中心体、着丝粒、有丝分裂纺锤体、中心颗粒体的底物蛋白，对有丝分裂多个阶段的正确进展起关键作用（2-4）。PLK1的PBD作为磷酸化丝氨酸/苏氨酸结合基序而发挥作用，识别最优识别基序Ser-(pSer/pThr)-(Pro/X)（5）。PLK1的PBD结合包含Ser-(pSer/pThr)-(Pro/X)基序的磷酸化肽段后解除其对激酶活性抑制功能（6）。Ser-(pSer/pThr)-Pro肽被脯氨酸导向的激酶如细胞周期依赖激酶（CDK）磷酸化。这些发现提示其它有丝分裂激酶如CDK对底物或接合蛋白可能是通过PLK1和其底物作用而同时激活其激酶活性而促发的。Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb是研究PLK1结合蛋白和PLK1底物的有用工具。

原厂资料：

Specificity / Sensitivity

Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb detects endogenous levels of cellular proteins containing the Ser-phospho-Thr-Pro motif. This antibody has minor cross-reactivity with phospho-Thr-Pro motifs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with an SpTP peptide library.

Background

Polo-like kinases (PLKs) are Ser/Thr protein kinases that play an essential role during the cell cycle. At least four PLKs exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4 (1). PLKs have a highly conserved amino-terminal kinase domain and a relatively divergent carboxy-terminal domain called the Polo-box domain (PBD). Of the four PLKs, PLK1 is the best characterized (2). PLK1 functions as a key regulator of mitotic events by phosphorylating substrate proteins on centrosomes, kinetochores, the mitotic spindle, and the midbody, and is crucial for proper progression through multiple stages of mitosis (2-4). The PBDs of PLK1 function as a phospho-Ser/Thr-binding module, recognizing the optimal recognition sequence motif Ser-(pSer/pThr)-(Pro/X) (5). Binding of phosphopeptides containing the Ser-(pSer/pThr)-(Pro/X) motif by the PBD in PLK1 relieves its inhibitory function on kinase activity (6). Ser-(pSer/pThr)-Pro peptides are phosphorylated by proline-directed kinases such as cyclin dependent kinases (CDKs). These findings imply that priming phosphorylations on substrates or docking proteins by other mitotic kinases such as CDKs may target PLK1 to its substrates and simultaneously activate its kinase activity. Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb is a useful tool to study PLK1 binding proteins and PLK1 substrates.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody