Mouse Interleukin-13 (mIL-13)

• 产品编号：CST-5242SC      品牌：CST       原厂货号：5242SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的小鼠重组蛋白IL-13 (mIL-13) Ser26-Phe131 (Accession #NP_032381)是从人的293细胞表达而来。重组的mIL-13 蛋白没有标签，未糖基化的蛋白分子量据推算为11,677Da。DTT-还原和未还原的蛋白分子作为12-20 kDa的蛋白而迁移。在SDS-PAGE中具有稍低的迁移率是由于糖基化。重组mIL-13蛋白的N-末端SVSLP的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) 重组mIL-13通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。mIL-13 的生物活性是通过B9细胞增殖实验来确定的。每个批次的ED50在0.8 - 4 ng/ml之间。

有载体: 每微克mIL-13蛋白溶解在包含 20 μg BSA的PBS(pH 7.2) 溶液中，并通过 0.22 μm 滤膜冻干。

无载体： 每微克mIL-13蛋白溶解在PBS(pH 7.2) 溶液中，并通过 0.22 μm 滤膜冻干。

IL-13由T细胞产生并且在TH2应答中非常重要。IL-13的目标包括B细胞、嗜酸性粒细胞、成纤维细胞、肥大细胞和巨噬细胞(1-3)。IL-13特异性的结合到IL-13Rα1并与IL-4Rα 形成II类 IL-4R复合体。Jak1和 Tyk2 的激活和信号转导是通过Stat3和Stat6 (6)。 IL-13Rα2是不同的基因产物，缺乏细胞内结构域，不与IL-4Rα形成复合体并且也不进行信号转导(1,6,7)。 IL-13Rα2的胞外域通常在疾病状态下升高表达。IL-13在气道高反应性(AHR)过敏性哮喘(1,4,5)和改变对寄生生物的抵抗中发挥重要作用(1)。

原厂资料：

Source / Purification

Recombinant mouse IL-13 (mIL-13) Ser26-Phe131 (Accession #NP_032381) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mIL-13 contains no "tags" and the nonglycosylated protein has a calculated MW of 11,677. DTT-reduced and non-reduced protein migrate as 12-20 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal SVSLP of recombinant mIL-13 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-13. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mIL-13 was determined in a B9 cell proliferation assay. The ED50 of each lot is between 0.8 - 4 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg mIL-13.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-13. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-13 is produced by T cells and is important in the TH2 response. IL-13 targets include B cells, eosinophils, fibroblasts, mast cells and macrophages (1-3). IL-13 binds specifically to IL-13Rα1 that complexes with IL-4Rα to form the Type II IL-4R. Jak1 and Tyk2 are activated and signal through Stat3 and Stat6 (4). IL-13Rα2 is a different gene product, lacks the intracellular domain, does not complex with IL-4Rα and does not signal (1,4,5). The extracellular domain of IL-13Rα2 is often elevated in diseased states. IL-13 plays key roles in airway hyperresponsiveness (AHR) of allergic asthma (1,6,7) and modulates resistance to parasitic organisms (1).

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.