Human Betacellulin (hBTC)

• 产品编号：CST-5235LC      品牌：CST       原厂货号：5235LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > EGF 表皮生长因子
• 应用分类：

描述：

CST在大肠杆菌中生产重组人蛋白Betacellulin (hBTC) Asp31-Tyr111 (Accession #P35070)。重组的hBTC蛋白在N-末端没有Met标签，其分子量据推算为8,984Da。DTT-还原和未还原的蛋白迁移时是作为一个14kDa的多肽而转移。重组hBTC蛋白的N-末端DGNST的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hBTC通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hBTC的生物活性是同过MCF 10A细胞的细胞增殖实验确定的。每个批次的ED50在0.6-1ng/ml之间。内毒素含量:<0.01 ng/1 μg hBTC。

有载体: 每微克hBTC蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hBTC蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。乙胞素 (BTC)，EGF家族成员的配体，是广泛表达的生长因子，它最初是从小鼠胰腺 beta细胞系的条件型培养基中发现鉴定(1)。BTC合成一个178个氨基酸的跨膜前体蛋白并被蛋白酶水解剪切为80个氨基酸的成熟蛋白(1)。人的BTC与小鼠BTC具有74%的相似度(1)。BTC能结合到并激活ErbB家族的受体酪氨酸激酶EGFR/ErbB1, HER2/ErbB2, HER3/ErbB3 和 HER4/ErbB4 (1,2)。BTC也能够结合到并激活EGFR/ErbB1和HER4/ErbB4同型二聚体(1)。配体-受体的相互作用导致了PI3K和Erk通路的激活(1)。在体外，BTC诱导了胰腺beta细胞系的增殖(2)。此外, 研究结果也表明了在啮齿动物的肥胖模型中BTC诱导beta细胞再生并增加了胰岛素的分泌(2-4)。

原厂资料：

Source / Purification

Recombinant human Betacellulin (hBTC) Asp31-Tyr111 (Accession #P35070) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hBTC does not have a Met at the amino terminus and has a calculated MW of 8,984. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal DGNST of recombinant hBTC was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hBTC. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hBTC was determined in a MCF 10A cell proliferation assay. The ED50 of each lot is between 0.6 and 1.0 ng/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hBTC. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Betacellulin (BTC), a member of the EGF family of ligands, is a widely expressed growth factor that was originally identified in conditioned medium of a mouse pancreatic beta cell line (1). BTC is synthesized as a 178 amino acid transmembrane precursor protein that is proteolytically cleaved into an 80 amino acid mature protein (1). Human BTC shares 74% amino acid sequence identity with mouse BTC (1). BTC can bind to and activate the ErbB family of receptor tyrosine kinases, EGFR/ErbB1, HER2/ErbB2, HER3/ErbB3, and HER4/ErbB4 (1,2). BTC can also bind to and activate EGFR/ErbB1 and HER4/ErbB4 homodimers (1). Ligand-receptor interactions result in the activation of the PI3K and Erk pathways (1). BTC induces the proliferation of pancreatic beta cell lines in vitro (2). Moreover, research studies have shown that BTC induces beta cell regeneration and increased insulin secretion in rodent models of diabetes (2-4).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.