Human FGF acidic (hFGF acidic)

• 产品编号：CST-5234SC      品牌：CST       原厂货号：5234SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > FGF 成纤维细胞生长因子
• 应用分类：

描述：

Cell Signaling Technology使用大肠杆菌表达人重组酸性FGF蛋白(hFGF acidic) Phe16-Asp155 (Accession #NP_000791)。重组的hFGF羧基端不含甲硫氨酸，分子量为12,835.DTT-还原和非还原状态下蛋白迁移速率与16 kDa的多肽相当。预测的重组hFGF氨基端序列通过氨基酸测序确认。6 μg还原和未还原的mEGF由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%。重组hFGF的生物活性通过NIH/3T3细胞增殖实验而确认。每一批次的ED50均在7-20 pg/ml之间。1ug 酸性hFGF蛋白所含内毒素低于0.01ng。带载体：每1 μg 酸性hFGF溶于含有20 μgBSA、pH7.2的PBS溶液经0.22 μm滤器过滤后冻干。无载体：溶于pH7.2的PBS溶液经0.22 μm滤器过滤获得冻干物。酸性FGF是一种成纤维细胞和内皮细胞的潜在生长因子（1）。酸性FGF涉及损伤修复，血管生长和发育（1）。酸性FGF由细胞通过内质网/高尔基体的非依赖机制分泌产生（1,2）。酸性FGF与硫酸肝素的结合能力对于它与FGF受体结合并激活下游信号是必须的（1-4）。有4种不同的FGF受体，每一种都有多个剪切异构体（1,3）。酸性FGF与大部分但不是所有的FGFR有高亲和性（1）。酸性FGF结合FGFR激活的型号通路包括ras-raf-MAPK, PLCγ/PKC, 和PI3K/Akt信号通路（1）。

原厂资料：

Source / Purification

Recombinant human FGF acidic (hFGF acidic) Phe16-Asp155 (Accession #NP_000791) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hFGF acidic does not have a Met on the amino terminus and has a calculated MW of 15,835. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides. The expected amino-terminal FNLPP of recombinant hFGF acidic was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hFGF acidic. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hFGF acidic was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 7-20 pg/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hFGF acidic. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

FGF acidic is a potent growth factor for fibroblasts and endothelial cells (1). FGF acidic is involved in wound repair, angiogenesis, and development (1). FGF acidic is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF acidic to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF acidic binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles