Mouse Transforming Growth Factor β1 (mTGF-β1)

• 产品编号：CST-5231LC      品牌：CST       原厂货号：5231LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TGF-beta 转化生长因子beta超家族
• 应用分类：

描述：

Cell Signaling Technology公司使用人293T细胞表达小鼠重组蛋白TGF-β1 (hTGF-β1) Ala279-Ser390 (编号 #NP_035707)。重组mTGF-β1蛋白不带标记且非糖基化的蛋白分子量为12,810。DTT-还原状态下蛋白迁移状态与13KDa多肽相同，非还原状态以半胱氨酸连接形成的同二聚体与25kDa蛋白相同。重组mTGF-β1氨基端序列ALDTN通过氨基酸测序确认。6 μg还原和未还原的mEGF由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%。mTGF-β1的活性通过检测其对IL-4诱导的HT-2细胞增殖的抑制能力来确定。带载体：每1 μg mTGF-β1溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的溶液经0.22 μm滤器过滤后冻干。无载体：经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物。TGF-β1的功能包括促进增殖，血管生成，和引发或抑制许多免疫活动（1-3）。TGF-β1可以被多种细胞生成，包括调节性T细胞，成纤维细胞，上皮细胞和内皮细胞（3）。TGF-β1与TβRII同二聚体结合，随后与TβRI同二聚体形成复合物（1，4）。这种低聚物受体复合物磷酸化Smad蛋白随后诱导或抑制一系列靶基因（1,3,4）。TGF-β1也可以通过TAK1激活Erk2，p38和JNK信号通路（4）。TGF-β1可以在某些癌症中促进晚期恶化和转移（1,2）。

原厂资料：

Source / Purification

Recombinant mouse TGF-β1 (hTGF-β1) Ala279-Ser390 (Accession #NP_035707) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mTGF-β1 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,810. DTT-reduced protein migrates as a 13 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 25 kDa protein. The expected amino-terminal ALDTN of recombinant mTGF-β1 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mTGF-β1. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mTGF-β1 was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 40-200 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mTGF-β1.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg mTGF-β1. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

TGF-β1 activities include proliferation, angiogenesis, and promotion or inhibition of many immune events (1-3). TGF-β1 is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells (3). TGF-β1 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,4). The oligomeric receptor complex phosphorylates subsets of the Smad proteins that then act to induce and repress a number of target genes (1,3,4). TGF-β1 binding can also activate the Erk2, p38, and JNK pathways via TAK1 (4). TGF-β1 appears to promote late stage progression and metastasis in some cancers (1,2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.