Mouse Macrophage Colony Stimulating Factor (mM-CSF)

• 产品编号：CST-5228LF      品牌：CST       原厂货号：5228LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > CSF集落刺激因子
• 应用分类：

描述：

CST公司在人293细胞中生产重组鼠蛋白M-CSF (mM-CSF) Lys33-Glu262 (Accession #NP_031804)。重组的mM-CSF蛋白在N-末端没有标签，未糖基化蛋白分子量据推算为25,987Da。DTT-还原的蛋白作为37-50kDa多肽而转移，未还原的蛋白经二硫键连接成同源二聚体迁移时是作为一个55-80 kDa的蛋白而转移，异源二聚体在SDS-PAGE胶中稍低的迁移率是由于糖基化。重组mM-CSF蛋白的N-末端KEVSE的序列通过测序确定。6 μg 还原 (+) 和 非还原的(-) mM-CSF通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白mM-CSF的生物活性是通过M-NFS-60细胞增值实验确定的。每个批次的ED50在0.5-2.0 ng/ml之间。内毒素含量:<0.01 ng /1 μg mM-CSF。

有载体: 每微克mM-CSF蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mM-CSF蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

GM-CSF 是通过活化的T细胞，NK细胞和巨噬细胞产生(1,2)。靶标细胞包括粒性白细胞，单核细胞前体和经过分化的一系列的骨髓细胞(1,3,4)。很多的靶标细胞需要GM-CSF才能生存。GM-CSF 诱导增殖，并参与造血细胞的分化成树状细胞，它在干细胞分化的信号通路上也是主要的因素之一。GM-CSF 激活骨髓细胞的功能，所以它连接天然免疫和获得性免疫并推进抗肿瘤免疫(5)。GM-CSF受体由GM-CSFRα和共有的 β链-βC 组成，这同时也被IL-3和 IL-5所利用(1)。GM-CSF的结合起始了 Jak2, Stat5 和 PI3K/Akt 信号通路(1)。

原厂资料：

Source / Purification

Recombinant mouse M-CSF (mM-CSF) Lys33-Glu262 (Accession #NP_031804) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mM-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 25,987. DTT-reduced protein migrates as a 37-50 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 55-80 kDa protein. Heterogeneity and lower mobility in SDS-PAGE are due to glycosylation. The expected amino-terminal KEVSE of recombinant mM-CSF was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mM-CSF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mM-CSF was determined in an M-NFS-60 cell proliferation assay. The ED50 of each lot is between 0.5-2.0 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mM-CSF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mM-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Macrophage-colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in M-CSF expression may contribute to cancer progression, and high plasma M-CSF levels are associated with rheumatoid arthritis (1,4,5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.