Mouse Interferon-γ (mIFN-γ)

• 产品编号：CST-5222SF      品牌：CST       原厂货号：5222SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > Interferon 干扰素
• 应用分类：

描述：

CST公司生产的鼠源重组蛋白IFN-γ (mIFN-γ) His23-Cys155 (Accession # NP_032363)是从大肠杆菌细胞表达而来。重组mIFN-γ蛋白在N-末端有Met标签，蛋白分子量据推算为15,652Da。DTT-还原蛋白和未还原蛋白作为14kDa的多肽迁移。重组mIFN-γ蛋白的N-末端MHGTV的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) 重组mIFN-γ通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。mIFN-γ的生物活性是通过病毒保护实验来确定的。每个批次的ED50在30-150 pg/ml之间。每微克mIFN-γ内毒素含量小于0.01 ng。

有载体：每微克mIFN-γ蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIFN-γ蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。IFN-γ在天然免疫和获得性免疫应答中发挥重要作用。IFN-γ激活天然免疫细胞如巨噬细胞和NK细胞的细胞毒活性(1,2)。通过NK细胞和具有抗原细胞(APCs)产生IFN-γ促进了细胞介导的获得性免疫，包括诱导T淋巴细胞产生IFN-γ、增加I型和II型 MHC 分子的表达、增强肽抗原呈递(1)。IFN-γ的抗病毒活性与PKR蛋白的诱导和其它的调控蛋白有关。IFN-γ结合到 IFNGR1/IFNGR2 复合体促进了受体的二聚化并形成(IFNGR1/IFNGR2)2 -IFN-γ二聚体。这种结合诱导了受体胞内域构象的改变并激活包括Jak1、 Jak2和 Stat1 的信号通路(3)。IFN-γ的重要作用是放大免疫监控，在IFN-γ或IFNGR敲除老鼠和IFNGR1或IFNGR2失活突变体中，病原菌感染的易感性增加。IFN-γ在动脉硬化中也发挥了重要的作用(4)。

原厂资料：

Source / Purification

Recombinant mouse IFN-γ (mIFN-γ) His23-Cys155 (Accession # NP_032363) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mIFN-γ has a Met on the amino terminus and has a calculated MW of 15,652. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminus MHGTV of recombinant mIFN-γ was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIFN-γ. All lots are greater than 98% pure.

Bioactivity

The bioactivity of mIFN-γ was determined in a virus protection assay. The ED50 of each lot is between 30-150 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIFN-γ.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIFN-γ. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen-presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increased class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice, and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles