Human β-Nerve Growth Factor (hβ-NGF)

• 产品编号：CST-5221SC      品牌：CST       原厂货号：5221SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > Neurotrophin神经营养因子
• 应用分类：

描述：

CST公司在人293细胞中生产重组人蛋白β-NGF (hβ-NGF) Ser122-Ala241 (Accession #NP_002497)。基于氨基酸测序结果，85%以上的重组hβ-NGF 蛋白在 Ser122 (SSSHP)位点起始，据推算分子量为 13,494Da。其余的蛋白在Ser124 (SHPIF)位点起始。DTT-还原和未还原蛋白作为13kDa的非二硫键连接的二聚体多肽而转移。6 μg 还原 (+) 和 非还原的(-) hβ-NGF通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hβ-NGF 的生物活性是通过TF-1细胞的细胞增殖实验确定的。每个批次的ED50在0.5-1.5 ng/ml之间。

有载体: 每微克hβ-NGF蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hβ-NGF蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

β-NGF是生长因子神经营养因子的经典成员之一(1)。β-NGF参与到神经元的存活、分化和生长(1,2)。在神经系统外围, NGF通过一系列的免疫细胞产生，包括B细胞、T细胞、单核细胞核柱状细胞(3,4)。β-NGF结合到两个不同的受体并TrkA和p75NTR上并引起信号(1,2)。NGF诱导的胞内响应通过受体表达而得到调节。例如, TrKA导致了凋亡的抑制和神经的分化。相反, 通过p75NTR的信号通路（在 TrkA不存在时）诱导细胞死亡(1,2)。NGF通过TrkA的信号的特点是对PI3K/AKT和PLCγ信号通路的激活(1,2)。NGF通过p75NTR的信号诱导 JNK和NFκB 的激活(1,2)。异常的NGF信号可能与阿尔茨海默病的发病有关(5,6)。

原厂资料：

Source / Purification

Recombinant human β-NGF (hβ-NGF) Ser122-Ala241 (Accession #NP_002497) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Based on amino acid sequencing, greater than 85% of recombinant hβ-NGF starts at Ser122 (SSSHP) and has a calculated MW of 13,494. The remainder starts at Ser124 (SHPIF). DTT-reduced and non-reduced protein migrate as 13 kDa non-disulfide linked homodimers.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hβ-NGF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hβ-NGF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 0.5-1.5 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hβ-NGF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hβ-NGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

β-NGF is the prototypical member of the neurotrophin family of growth factors (1). β-NGF is involved in neuronal survival, differentiation and growth (1,2). Outside of the nervous system, NGF is produced by a variety of immune cells, including B cells, T cells, monocytes and mast cells (3,4). β-NGF binds to and signals through two distinct receptors, TrkA and p75NTR (1,2). Cellular responses induced by NGF are modulated by receptor expression. For example, TrKA leads to the inhibition of apoptosis and neuronal differentiation. In contrast, signaling through p75NTR in the absence of TrkA induces cell death. (1,2). NGF signaling via TrkA is characterized by activation of the PI3K/AKT and PLCγ pathways (1,2). NGF signaling via p75NTR induces JNK and NFκB activation (1,2). Aberrant NGF signaling may be linked to the onset of Alzheimer’s disease (5,6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles