Human Neuregulin-1 (hNRG-1)

• 产品编号：CST-5218SC      品牌：CST       原厂货号：5218SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > EGF 表皮生长因子
• 应用分类：

描述：

CST公司在大肠杆菌中生产重组人源蛋白NRG-1 (hNRG-1) Thr176-Lys238 (Accession #NP_001153480)。重组的hNRG-1蛋白在N-末端没有Met，其分子量据推算为7,415Da。DTT-还原和未还原的蛋白迁移时是作为一个6 kDa 的多肽而转移。重组hNRG-1蛋白的N-末端TSHLV的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hNRG-1通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hNRG-1的生物活性是同过MCF7细胞的细胞增殖实验确定的。每个批次的ED50在1-4 ng/ml之间。

有载体: 每微克hNRG-1蛋白溶解在包含 20 μg BSA的20 mM 柠檬酸盐（pH 3.0）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hNRG-1蛋白溶解在20 mM 柠檬酸盐（pH 3.0）溶液中，并通过 0.22 μm 滤膜冻干。

NRG-1是EGF家族成员之一, 它参与了心脏、乳腺和神经系统的发育(1)。NRG-1是通过乳腺上皮细胞、血管内皮细胞核神经元细胞表达 (2,3)。至少有15种 NRG-1可变剪切是已知的(1)。这些不同的剪切形式差别主要是在EGF域(α或 β形式)、N-末端的可变剪切位点和结合外显子编码整合膜域(1)。NRG-1能够抑制乳腺癌起源细胞的增殖。NRG-1不同的作用可能依赖于不同的剪切形式以及与受体的相互作用(2)。NRG-1结合到ErbB3/HER3或者ErbB4/HER4。结合又诱导了与 ErB2/HER2的二聚化。Akt, Erk1/2和Erk5 通路也参与到了NRG-1激活的信号过程中(4,5)。NRG-1可能对精神分裂症和乳腺癌中也有作用(1,4,6)。

原厂资料：

Source / Purification

Recombinant human NRG-1 (hNRG-1) Thr176-Lys238 (Accession #NP_001153480) was produced in E. coli cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hNRG-1 does not have a Met on the amino terminus and has a calculated MW of 7,415. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal TSHLV of recombinant hNRG-1 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNRG-1. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hNRG-1 was determined in a cell proliferation assay using MCF7 cells. The ED50 of each lot is between 1-4 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hNRG-1.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hNRG-1. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

NRG-1, a member of the EGF family, is involved in heart, mammary and nervous system development (1). NRG-1 is expressed by mammary epithelial, vascular endothelial and neuronal cells (2,3). At least 15 NRG-1 splice variants are known (1). These variants differ in EGF domains (α or β variants), amino-terminal splicing sites, and incorporation of exons encoding integral membrane regions (1). NRG-1 can induce or inhibit the proliferation of cells derived from breast cancer. The differences in effects of NRG-1 appear to depend on splice variation and interaction with receptor(s) (2). NRG-1 binds to ErbB3/HER3 or ErbB4/HER4. Binding induces dimerization with ErB2/HER2. The Akt, Erk1/2 and Erk5 pathways have been shown to participate in NRG-1 activated signaling (4,5). NRG-1 appears to have roles in schizophrenia and breast cancer (1,4,6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.