Mouse Interleukin-7 (mIL-7)

• 产品编号：CST-5217SC      品牌：CST       原厂货号：5217SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司在大肠杆菌中生产重组鼠蛋白IL-7 (mIL-7) Glu26-Ile154 (Accession #NP_032397)。重组的hIL-8蛋白在N-末端有Met标签，分子量据推算为15,028Da。DTT-还原和未还原的蛋白迁移时是作为一个14kDa 的多肽而转移，重组mIL-7蛋白的N-末端MECHI的序列通过测序确定。6 μg 还原 (+) 和 非还原的(-) mIL-7通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白mIL-7的生物活性是通过2E8细胞增值实验确定的。每个批次的ED50在60-200 pg/ml之间。内毒素含量:<0.01 ng /1 μg mIL-7。

有载体: 每微克mIL-7蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIL-7蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-7 在淋巴细胞增殖和淋巴细胞的动态平衡中发挥了重要的作用(1)。在骨髓和胸腺中的基质和上皮细胞产生IL-7(1)。IL-7最主要的靶标是T细胞、B细胞和树突细胞(1)。IL-7对T细胞的发育非常重要，此重要性在缺失T细胞的小鼠和人中的IL-7/IL-7R信号缺陷中被低估(1,2)。同时，在小鼠中IL-7是B细胞的发育中必需的, IL-7在人的B细胞发育中的作用尚不清楚(1,3)。除了它在T淋巴细胞增殖中的影响, IL-7促进空白和记忆αβ T和γδ T细胞的维持和存活(1)。IL-7的受体是共有的γ链-γc和 IL-7特异的IL-7Rα构成的异质二聚体(1)。IL-7激活PI3K/Akt, Jak1/2 和 Stat1, 3和 5 (1)。

原厂资料：

Source / Purification

Recombinant mouse IL-7 (mIL-7) Glu26-Ile154 (Accession #NP_032397) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mIL-7 has a Met on the amino terminus and has a calculated MW of 15,028. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal MECHI of recombinant mIL-7 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-7. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 60-200 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-7.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-7 plays key a role in lymphopoiesis, and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory α/β T cells as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7 specific IL-7Rα(1). IL-7 activates PI3K/Akt, Jak 1/2, and Stat 1, 3, and 5 (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.