Mouse Interleukin-1β (mIL-1β)

• 产品编号：CST-5204SF      品牌：CST       原厂货号：5204SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-1 Family 白细胞介素-1超家族
• 应用分类：

描述：

CST公司生产的鼠源重组蛋白IL-1β (mIL-1β) Val118-Ser269 (Accession #NP_032387)是从大肠杆菌细胞表达而来。重组的 mIL-1β 蛋白在N-末端有Met标签，其蛋白分子量据推算为17,525Da。DTT-还原和未还原的蛋白作为一个18kDa的蛋白转移。重组mIL-1β蛋白的N-末端MVPIR的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) mIL-1β通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组的mIL-1β生物活性是通过诱导3T3 MEFs WT细胞产生IL-6的能力来确定的。每个批次的ED50在5-20 pg/ml之间。IL-1β是促炎症因子，主要的由激活的单核细胞和上皮细胞产生(1)。IL-1β的前体被caspase-1剪切，成熟的IL-1β紧接着被分泌(1-3)。目标细胞包括巨噬细胞和许多其它的细胞类型。IL-1β引起的信号通路包括IL-1β结合到IL-1的附属蛋白 (IL-1-AcP); 此复合体紧接着结合到IL-1RI (1,2)。信号通过激活MAP激酶和 NF-κB信号通路(1,2)。IL-1β也结合到IL-1RII,它缺乏细胞内的信号区域并因此作为高亲和性的诱捕受体。IL-1β结合到IL-1RI的过程能够被负调控因子IL-1R拮抗剂IL-1Ra所抑制。IL-1Ra结合到IL-1RI后无信号传导并且作为抑制剂阻止IL-1β信号通路。IL-1β在急性应答期和脓毒病中发挥了重要的作用(1-3)。

原厂资料：

Source / Purification

Recombinant mouse IL-1β (mIL-1β) Val118-Ser269 (Accession #NP_032387) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mIL-1β has a Met on the amino terminus and has a calculated MW of 17,525. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal MVPIR of recombinant mIL-1β was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-1β. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mIL-1β was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 5-20 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-1β.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-1β. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-1β is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.