Lipin 1 Antibody

• 产品编号：CST-5195S      品牌：CST       原厂货号：5195S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lipin 1 Antibody用于检测内源性Lipin 1总蛋白水平。多克隆抗体是通过合成对应于人Lipin 1蛋白 Gln883 位点肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。脂质1最初作为脂肪组织分化所必需的一个核蛋白被发现（1）。在脂肪细胞的分化过程中，Lipin 1被诱导表达（1）。Lipin 1的突变会导致脂肪组织的异常发育，从而导致脂质代谢失调，引起低体脂症，脂肪肝，高甘油三酯血症和胰岛素抵抗（1）。Lipin 1在多种组织和细胞如肝脏，肌肉，脂肪和神经细胞的脂质代谢中发挥作用（2-4）。Lipin 1在分子层面有双重作用，首先，它作为肝脏中的一个辅转录因子，其次，它是甘油三酯和磷脂生物合成通路中的一个磷脂磷酸酶（5）。Lipin 1由mToR通路调控，这提示着脂质发育和营养感受通路之间有着联系（6）。它还可以通过TORC2/CRTC2调控肝脏的胰岛素信号（7）。

1. Péterfy, M. et al. (2001) Nat Genet 27, 121-4.
2. Finck, B.N. et al. (2006) Cell Metab 4, 199-210.
3. Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83.
4. Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64.
5. Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6.
6. Huffman, T.A. et al. (2002) Proc Natl Acad Sci U S A 99, 1047-52.
7. Ryu, D. et al. (2009) Cell Metab 9, 240-51.

原厂资料：

Specificity / Sensitivity

Lipin 1 Antibody detects endogenous levels of total Lipin 1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln883 of human Lipin 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lipin 1 was identified as a nuclear protein required for adipose tissue development (1). The expression of Lipin 1 is induced during adipocyte differentiation (1). The abnormal development of adipose tissues caused by mutations in the lipin 1 gene results in lipodystrophy, a condition associated with low body fat, fatty liver, hypertriglyceridemia, and insulin resistance (1). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (2-4). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in liver, and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (5). Lipin1 is regulated by mTOR, illustrating a connection between adipocyte development and nutrient-sensing pathways (6). It also mediates hepatic insulin signaling by TORC2/CRTC2 (7).

1. Péterfy, M. et al. (2001) Nat Genet 27, 121-4.
2. Finck, B.N. et al. (2006) Cell Metab 4, 199-210.
3. Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83.
4. Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64.
5. Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6.
6. Huffman, T.A. et al. (2002) Proc Natl Acad Sci U S A 99, 1047-52.
7. Ryu, D. et al. (2009) Cell Metab 9, 240-51.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody