Phospho-IRF-7 (Ser471/472) Antibody

• 产品编号：CST-5184S      品牌：CST       原厂货号：5184S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-IRF-7 (Ser471/472) 抗体能够检测内源在Ser471和472位点磷酸化的IRF-7蛋白。此多克隆抗体是通过合成人源对应的IRF-7 Ser471/472位点周围的磷肽来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。干扰素调控因子(IRFs)由一个家族的转录调控因子组成，这个家族在 Jak/Stat信号通路中的作用是，在病毒感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自体免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥重要作用。IRF家族包含9个成员：IRF-1、IRF-2、ISGF3γ/p48、IRF-3、IRF-4 (Pip/LSIRF/ICSAT)、IRF-5、IRF-6、IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都具有同源性。IRF家族成员通过与具有相似基序的DNA-结合区域的蛋白相互作用来调控转录，例如IFN刺激的应答元件(ISRE)、IFN consensus序列 (ICS) 和IFN 调控元件(IRF-E) (2)。IRF-7在功能上与IRF-3相似，优先在淋巴样细胞中表达，并且能被病毒、LPS 和 IFN-α所诱导(3-5)。受到病毒感染后，IRF-7在诱导I型干扰素表达方面发挥了重要的作用(6-8)。与IRF-3相似, IRF-7的调控是通过在C-末端附近多个丝氨酸位点磷酸化而实现，这对其核定位，DNA结合和转录活性都是必需的(9-11)。

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Au, W.C. et al. (1998) J Biol Chem 273, 29210-7.
4. Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18.
5. Marié, I. et al. (1998) EMBO J 17, 6660-9.
6. Sato, M. et al. (2000) Immunity 13, 539-48.
7. Honda, K. et al. (2005) Nature 434, 772-7.
8. Colina, R. et al. (2008) Nature 452, 323-8.
9. Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
10. Yang, H. et al. (2003) J Biol Chem 278, 15495-504.
11. Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7.

原厂资料：

Specificity / Sensitivity

Phospho-IRF-7 (Ser471/472) Antibody detects transfected levels of IRF-7 when phosphorylated at Ser471 and 472.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser471/472 of human IRF-7 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response viral infection (6-8). Like IRF-3, IRF-7 is regulated at multiple serine phosphorylation sites near its carboxyl terminus, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11).

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Au, W.C. et al. (1998) J Biol Chem 273, 29210-7.
4. Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18.
5. Marié, I. et al. (1998) EMBO J 17, 6660-9.
6. Sato, M. et al. (2000) Immunity 13, 539-48.
7. Honda, K. et al. (2005) Nature 434, 772-7.
8. Colina, R. et al. (2008) Nature 452, 323-8.
9. Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
10. Yang, H. et al. (2003) J Biol Chem 278, 15495-504.
11. Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.